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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2008, 76(1), 65-76; https://doi.org/10.3797/scipharm.0802-03 (registering DOI)

Illicium verum Hook. f. and Illicium anisatum L.: Anatomical Characters and their Value for Differentiation

1
University of Vienna, Department of Pharmacognosy, Althanstrasse 14, A-1090 Vienna, Austria
2
Austrian Medicines and Medical Devices Agency AGES PharmMed, Schnirchgasse 9, A-1030 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 24 January 2008 / Accepted: 26 February 2008 / Published: 30 March 2008
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Abstract

In the last years intoxications after consumption of Illicium verum Hook. f. have been reported due to adulterations with I. anisatum L.. Many efforts have already been made to develop a simple method which would enable the detection of I. anisatum in powdered samples of I. verum. Since many controversial facts concerning the value of anatomic characters for differentiation have been published 13 botanically vouchered samples were re-examined by means of light microscopy, fluorescence microscopy, SEM, and confocal laser scanning microscopy. All anatomical details show a great variability even between the follicles of a certain aggregate. This is also true for the cuticular striation which has been proposed in literature as reliable character. Special attention was paid to the structure of the aleurone grains of the endosperm and the shape of the calcium oxalate crystals in the testa. In contrast to previous assumptions these characters do not allow a certain differentiation either. However, the presence of hexagonal crystals can serve as indicator for samples of I. anisatum. At present a reliable proof of purity of powdered samples of I. verum seems to be possible by chemical analytical methods only.
Keywords: Illicium verum; Illicium anisatum; differentiation; microscopy; anatomical characterization Illicium verum; Illicium anisatum; differentiation; microscopy; anatomical characterization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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FRITZ, E.; ÖLZANT, S.M.; LÄNGER, R. Illicium verum Hook. f. and Illicium anisatum L.: Anatomical Characters and their Value for Differentiation. Sci. Pharm. 2008, 76, 65-76.

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