Spectrophotometric Determination of Aciclovir, Cefepirne Hydrochloride, Etamsylate and Metoclopramide Hydrochloride Usina 1,10 Phenanttrroline—Fe(lll) Reaqent

A simple, rapid, sensitive and accurate method for the determination of aciclovir, cefepime HCI, etamsylate and metoclopramide HCI in pure form and in pharmaceutical formulations is developed.The method is based on the fomabon of tris(o-phenanthroline) iron(ll) complex (Ferroin) upon the reaction of the ated drugs wrth iron(lll )-o- phenanthroline mixture. The ferroin complex is colorimetncally measured at λ_max 510 nm against a reagent blank. 0ptimization of the experimental conditions is described. Beer's law is obeyed in the concentration range from 0.25–30 µg ml⁻¹ with molar absorpitivities (ε) ranging from 4.796 x 10³–9.51 2 x 10⁴ L.mol⁻¹.cm⁻¹ and Sandell sensitivities (S) of 2.129 x 10⁻³–34.5 x 10⁻³ µg cm⁻². The developed method is applied successfully for the determination of the cited drugs in pure forms and in the corresponding pharmaceutical formulations without any interferences from common excipients.