Loss of SMYD1 Results in Perinatal Lethality via Selective Defects within Myotonic Muscle Descendants
Abstract
:1. Introduction
2. Methods and Materials
2.1. Generation of Myog-Cre/Smyd1flox Mice
2.2. Mating Scheme
2.3. PCR Genotyping
2.4. Recombination Assays
2.5. In Situ Hybridization
2.6. Protein/Immunohistochemistry
2.7. End-Point RT-PCR
2.8. RT-qPCR
2.9. RNA In Situ Hybridization
3. Results and Discussion
3.1. MyoG-Cre Deletion is Specific to Skeletal Muscle
3.2. MyoG-Cre-Mediated Smyd1 CKO Results in Perinatal Lethality
3.3. Skeletal Muscle Development in the MyoG-Cre-Induced Smyd1 CKO is Significantly Derailed
3.4. MyoG-Cre-Induced Smyd1 CKOs Exhibit Proliferative Defects and Non-Autonomous Deposits of Brown Adipose Tissue
3.5. Skeletal Muscle SMYD1 Deficiency Does not Affect Transcript Levels of Myogenic Regulatory Factors
3.6. Central Regulators of BAT are Unaffected by SMYD1 CKO
3.7. SMYD1 Regulates Immune Factors Critical for BAT Development and Physiology
3.8. SMYD1 as a Potential Determinant of Muscle vs. Adipose Fate
3.9. SMYD1 Controls Specification of Muscle Fiber-Type in Rare Adult Survivors
4. Concluding Remarks
Supplementary Materials
Author Contributions
Funding
Acknowledgements
Conflicts of Interest
References
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Age (DPC) | Smyd1 MT | Smyd1 HET | Smyd1 WT | Total |
---|---|---|---|---|
9.5 | 5 | 11 | 6 | 22 |
12.5 | 4 | 9 | 10 | 23 |
13.5 | 2 | 3 | 3 | 8 |
14.5 | 0 | 4 | 0 | 4 |
15.5 | 6 | 11 | 8 | 25 |
16.5 | 6 | 5 | 2 | 13 |
17.5 | 2 | 4 | 3 | 9 |
Total | 25 | 47 | 32 | 104 |
Expected Ratio | 0.25 | 0.5 | 0.25 | |
Observed Ratio | 0.24 | 0.45 | 0.31 |
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Rasmussen, T.L.; Tucker, H.O. Loss of SMYD1 Results in Perinatal Lethality via Selective Defects within Myotonic Muscle Descendants. Diseases 2019, 7, 1. https://doi.org/10.3390/diseases7010001
Rasmussen TL, Tucker HO. Loss of SMYD1 Results in Perinatal Lethality via Selective Defects within Myotonic Muscle Descendants. Diseases. 2019; 7(1):1. https://doi.org/10.3390/diseases7010001
Chicago/Turabian StyleRasmussen, Tara L., and Haley O. Tucker. 2019. "Loss of SMYD1 Results in Perinatal Lethality via Selective Defects within Myotonic Muscle Descendants" Diseases 7, no. 1: 1. https://doi.org/10.3390/diseases7010001