Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development
Abstract
:1. Introduction
2. Experimental Section
2.1. Reagents
2.2. Kalanchoe pinnata Extract
2.3. Semi-Preparative HPLC Fractionation
2.4. Mass Spectrometry
2.5. Antibacterial Assay
2.6. Cell Culture
2.7. Skin Cell Viability Test
2.8. Antioxidant Assay on Normal Human Epidermis Keratinocytes
3. Results and Discussion
3.1. Semi-Preparative HPLC Fractionation
3.2. Antibacterial Assay
Samples | Ec | Sa | Pa | Ca | Ab |
---|---|---|---|---|---|
CE 66 µg mL−1 | − | ++ | − | ++ | − |
CE 132 µg mL−1 | + | + | − | ++ | − |
CE 264 µg mL−1 | + | + | +/− | + | + |
Fraction 1 | + | + | − | +/− | − |
Fraction 2 | + | + | − | +/− | − |
Fraction 3 | ++ | + | +/− | +/− | − |
Fraction 4 | − | + | +/− | +/− | − |
Fraction 5 | − | + | − | +/− | − |
Fraction 6 | +/− | + | − | +/− | − |
Fraction 7 | − | + | − | ++ | − |
Fraction 8 | − | + | + | ++ | − |
Fraction 9 | + | + | + | ++ | − |
Fraction 10 | + | + | − | +/− | − |
Fraction 11 | − | ++ | − | +/− | − |
Fraction 12 | − | ++ | − | +/− | − |
Fraction 13 | + | ++ | − | +/− | − |
Fraction 14 | − | ++ | − | ++ | − |
Fraction 15 | ++ | − | − | ++ | − |
Fraction 16 | ++ | + | + | ++ | − |
Fraction 17 | +/− | + | + | +/− | − |
Fraction 18 | + | + | + | +/− | − |
Fraction 19 | − | + | − | +/− | − |
Fraction 20 | − | + | − | ++ | − |
Fraction 21 | − | + | − | ++ | − |
Fraction 22 | − | + | − | ++ | − |
Fraction 23 | + | ++ | − | +/− | − |
Fraction 24 | + | + | − | +/− | − |
Fraction 25 | + | + | + | ++ | − |
3.3. Viability Test
3.4. Antioxidant Assay on Normal Human Epidermis Keratinocytes
3.5. Mass Spectrometry
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Destandau, E.; Krolikiewicz-Renimel, I.; El Abdellaoui, S.; Cancellieri, P.; Fougère, L.; Toribio, A.; Landemarre, L.; André, P.; Elfakir, C. Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development. Cosmetics 2014, 1, 14-28. https://doi.org/10.3390/cosmetics1010014
Destandau E, Krolikiewicz-Renimel I, El Abdellaoui S, Cancellieri P, Fougère L, Toribio A, Landemarre L, André P, Elfakir C. Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development. Cosmetics. 2014; 1(1):14-28. https://doi.org/10.3390/cosmetics1010014
Chicago/Turabian StyleDestandau, Emilie, Isabelle Krolikiewicz-Renimel, Saida El Abdellaoui, Perrine Cancellieri, Laetitia Fougère, Alix Toribio, Ludovic Landemarre, Patrice André, and Claire Elfakir. 2014. "Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development" Cosmetics 1, no. 1: 14-28. https://doi.org/10.3390/cosmetics1010014