Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil
Department of Environmental Chemistry and Technology, Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic
Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
Department of Biology, Faculty of Science, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic
Author to whom correspondence should be addressed.
Received: 11 August 2020 / Revised: 18 September 2020 / Accepted: 20 September 2020 / Published: 22 September 2020
Marginal land represents poor soil with low agricultural characteristics and crop productivity, which is sometimes additionally contaminated. The exploitation of marginal land for normal agriculture is not possible but it suitable for cultivation of energy crops, especially Miscanthus × giganteus (Mxg), however, the harvest biomass value in such land is lower. The produced Mxg biomass can be converted to alternative energy like biofuel and biogas, or used for production of other value-added products like insulation fibers, building materials or paper, etc. It is well known fact that plant growth-promoting bacteria are beneficial for stimulating the overall development of plants even under stress conditions. In the current study, a number of strains were isolated from the metal-contaminated post-mining land, identified, biochemically characterized, and evaluated for abiotic stress tolerance: pH, temperature, salinity, and heavy metal (lead). Among different isolates, the multiple abiotic stress-tolerant plant growth-promoting bacteria Bacillus altitudinis KP-14 showed the best properties. Its effect on the growth of Mxg under the severe stress of metal-contaminated soil was evaluated. It was found that selected bacterial strain KP-14 significantly enhanced the biomass production. The overall results suggested that B. altitudinis KP-14 can be recommended as a potent biofertilizer for marginal lands.