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Article

Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil

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Department of Environmental Chemistry and Technology, Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic
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Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
3
Department of Biology, Faculty of Science, Jan Evangelista Purkyně University in Ústí nad Labem, 40096 Ústí nad Labem, Czech Republic
*
Author to whom correspondence should be addressed.
Biology 2020, 9(9), 305; https://doi.org/10.3390/biology9090305
Received: 11 August 2020 / Revised: 18 September 2020 / Accepted: 20 September 2020 / Published: 22 September 2020
Marginal land represents poor soil with low agricultural characteristics and crop productivity, which is sometimes additionally contaminated. The exploitation of marginal land for normal agriculture is not possible but it suitable for cultivation of energy crops, especially Miscanthus × giganteus (Mxg), however, the harvest biomass value in such land is lower. The produced Mxg biomass can be converted to alternative energy like biofuel and biogas, or used for production of other value-added products like insulation fibers, building materials or paper, etc. It is well known fact that plant growth-promoting bacteria are beneficial for stimulating the overall development of plants even under stress conditions. In the current study, a number of strains were isolated from the metal-contaminated post-mining land, identified, biochemically characterized, and evaluated for abiotic stress tolerance: pH, temperature, salinity, and heavy metal (lead). Among different isolates, the multiple abiotic stress-tolerant plant growth-promoting bacteria Bacillus altitudinis KP-14 showed the best properties. Its effect on the growth of Mxg under the severe stress of metal-contaminated soil was evaluated. It was found that selected bacterial strain KP-14 significantly enhanced the biomass production. The overall results suggested that B. altitudinis KP-14 can be recommended as a potent biofertilizer for marginal lands.
Use of plant growth-promoting bacteria (PGPB) for cultivation of the biofuel crop Miscanthus × giganteus (Mxg) in post-military and post-mining sites is a promising approach for the bioremediation of soils contaminated by metals. In the present study, PGPB were isolated from contaminated soil and screened for tolerance against abiotic stresses caused by salinity, pH, temperature, and lead (Pb). Selected strains were further assessed and screened for plant growth-promoting attributes. The isolate showing the most potential, Bacillus altitudinis KP-14, was tested for enhancement of Mxg growth in contaminated soil under greenhouse conditions. It was found to be highly tolerant to diverse abiotic stresses, exhibiting tolerance to salinity (0–15%), pH (4–8), temperature (4–50 °C), and Pb (up to 1200 ppm). The association of B. altitudinis KP-14 with Mxg resulted in a significant (p ≤ 0.001) impact on biomass enhancement: the total shoot and dry root weights were significantly enhanced by 77.7% and 55.5%, respectively. The significant enhancement of Mxg biomass parameters by application of B. altitudinis KP-14 strongly supports the use of this strain as a biofertilizer for the improvement of plant growth in metal-contaminated soils. View Full-Text
Keywords: abiotic stress; lead tolerance; Bacillus altitudinis; P-solubilization; Miscanthus × giganteus; post-mining metal-contaminated soil abiotic stress; lead tolerance; Bacillus altitudinis; P-solubilization; Miscanthus × giganteus; post-mining metal-contaminated soil
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MDPI and ACS Style

Pranaw, K.; Pidlisnyuk, V.; Trögl, J.; Malinská, H. Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil. Biology 2020, 9, 305. https://doi.org/10.3390/biology9090305

AMA Style

Pranaw K, Pidlisnyuk V, Trögl J, Malinská H. Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil. Biology. 2020; 9(9):305. https://doi.org/10.3390/biology9090305

Chicago/Turabian Style

Pranaw, Kumar, Valentina Pidlisnyuk, Josef Trögl, and Hana Malinská. 2020. "Bioprospecting of a Novel Plant Growth-Promoting Bacterium Bacillus altitudinis KP-14 for Enhancing Miscanthus × giganteus Growth in Metals Contaminated Soil" Biology 9, no. 9: 305. https://doi.org/10.3390/biology9090305

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