Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Suppliers
2.2. Animals and Ejaculates
2.3. Experimental Design
2.4. Analysis of Sperm Motility
2.5. Flow Cytometry Analyses
2.5.1. General Information about Flow Cytometry Analyses
2.5.2. Plasma Membrane Integrity
2.5.3. Evaluation of Mitochondrial Membrane Potential (∆Ψm, JC1)
2.5.4. DNA Integrity (SCSA Test)
2.6. Determination of Intracellular ATP Levels
2.7. Determination of O2 Consumption Rate
2.8. Statistical Analyses
3. Results
3.1. Effects of Red-Light Irradiation on Sperm Viability
3.2. Effects of Red-Light Irradiation on Mitochondrial Membrane Potential (ΔΨm)
3.3. Effects of Red-Light Irradiation on Sperm Motility
3.4. Sperm Subpopulations
3.5. Effects of Red-Light Stimulation on Intracellular ATP Levels
3.6. Effects of Red-Light Stimulation on Oxygen Consumption
3.7. Effects of Red-Light Stimulation on DNA Fragmentation
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Patterns | VCL (µm/s) | VSL (µm/s) | VAP (µm/s) | LIN (%) | STR (%) | WOB (%) | ALH (µm) | BCF (Hz) |
---|---|---|---|---|---|---|---|---|
Control | 107.2 ± 5.9 a | 56.9 ± 2.5 a | 81.9 ± 4.7 a | 55.6 ± 4.7 a | 71.0 ± 4.6 a | 77.6 ± 2.1 a | 2.7 ± 0.1 a | 9.7 ± 0.6 a |
Ph 1 | 116.0 ± 8.3 a | 58.9 ± 3.9 a | 88.5 ± 4.7 a | 52.2 ± 6.2 a | 66.3 ± 5.7 a | 77.2 ± 2.8 a | 2.9 ± 0.2 a | 9.3 ± 0.4 a |
Ph 2 | 117.2 ± 4.8 a | 62.3 ± 5.3 a | 92.0 ± 4.9 a | 53.6 ± 4.8 a | 68.0 ± 4.2 a | 78.1 ± 2.6 a | 2.9 ± 0.1 a | 9.1 ± 0.3 a |
Ph 3 | 113.2 ± 7.0 a | 60.7 ± 4.0 a | 90.4 ± 6.3 a | 54.5 ± 4.2 a | 68.2 ± 4.5 a | 79.6 ± 2.1 a | 2.9 ± 0.2 a | 9.1 ± 0.2 a |
Ph 4 | 117.7 ± 5.2 a | 62.5 ± 4.5 a | 94.4 ± 4.6 b | 53.2 ± 4.6 a | 66.2 ± 4.8 a | 80.2 ± 2.2 a | 3.0 ± 0.3 a | 9.2 ± 0.8 a |
Ph 5 | 111.3 ± 6.6 a | 58.9 ± 2.6 a | 87.7 ± 4.3 a | 54.8 ± 5.0 a | 68.6 ± 5.0 a | 79.2 ± 2.0 a | 2.6 ± 0.1 a | 9.4 ± 0.5 a |
Ph 10 | 115.4 ± 6.9 a | 62.6 ± 3.3 a | 91.8 ± 6.3 a | 55.4 ± 4.2 a | 69.4 ± 4.3 a | 79.5 ± 2.0 a | 2.9 ± 0.1 a | 9.3 ± 0.2 a |
Ph 1-1-1 | 112.4 ± 8.5 a | 60.7 ± 2.5 a | 84.6 ± 5.7 a | 55.0 ± 5.6 a | 70.6 ± 5.3 a | 77.0 ± 2.3 a | 3.2 ± 0.3 a | 9.1 ± 0.7 a |
Ph 2-1-2 | 116.3 ± 7.4 a | 62.9 ± 3.3 a | 90.6 ± 4.5 a | 56.1 ± 5.5 a | 70.6 ± 5.0 a | 78.6 ± 2.4 a | 3.2 ± 0.3 a | 8.8 ± 0.6 a |
Ph 2-2-2 | 121.6 ± 7.3 a | 62.4 ± 3.4 a | 96.0 ± 4.7 b | 53.5 ± 5.0 a | 65.9 ± 4.6 a | 79.6 ± 2.5 a | 2.9 ± 0.3 a | 8.6 ± 0.8 a |
Ph 3-1-3 | 115.6 ± 6.8 a | 61.1 ± 3.5 a | 91.4 ± 6.2 a | 53.7 ± 3.5 a | 68.2 ± 4.1 a | 78.6 ± 1.8 a | 3.3 ± 0.3 a | 8.6 ± 0.6 a |
Ph 3-3-3 | 119.5 ± 6.9 a | 65.7 ± 4.8 b | 96.4 ± 5.6 b | 55.6 ± 4.8 a | 68.0 ± 4.7 a | 79.7 ± 2.5 a | 3.1 ± 0.3 a | 8.4 ± 0.6 b |
Ph 4-1-4 | 112.3 ± 7.7 a | 63.9 ± 2.8 a | 93.3 ± 5.7 a | 59.6 ± 4.5 a | 72.6 ± 5.0 a | 81.9 ± 1.4 a | 2.8 ± 0.1 a | 9.6 ± 0.3 a |
Ph 4-4-4 | 112.0 ± 7.6 a | 64.4 ± 3.2 a | 94.8 ± 3.9 b | 59.0 ± 4.3 a | 71.6 ± 4.7 a | 82.2 ± 1.7 a | 2.6 ± 0.1 a | 9.0 ± 0.5 a |
Ph 5-1-5 | 113.0 ± 6.8 a | 64.0 ± 2.0 a | 91.0 ± 4.1 a | 58.1 ± 4.0 a | 71.2 ± 3.8 a | 81.1 ± 1.9 a | 3.1 ± 0.3 a | 8.4 ± 0.6 b |
Ph 5-5-5 | 112.4 ± 7.4 a | 66.7 ± 2.0 b | 92.7 ± 4.3 a | 61.3 ± 4.4 a | 74.4 ± 4.4 a | 82.4 ± 1.5 a | 2.9 ± 0.1 a | 8.9 ± 0.3 a |
Ph 10-5-10 | 105.8 ± 8.3 a | 61.3 ± 2.5 a | 86.0 ± 5.4 a | 60.8 ± 5.4 a | 73.7 ± 5.8 a | 81.9 ± 1.7 a | 2.5 ± 0.1 a | 9.1 ± 0.3 a |
Ph 10-10-10 | 109.4 ± 12.3 a | 58.1 ± 5.1 a | 84.0 ± 8.8 a | 57.3 ± 6.5 a | 73.0 ± 6.9 a | 77.4 ± 3.4 a | 2.8 ± 0.3 a | 9.7 ± 0.6 a |
Ph 15-5-15 | 109.2 ± 8.9 a | 60.5 ± 3.4 a | 83.6 ± 4.9 a | 58.6 ± 6.2 a | 74.3 ± 6.1 a | 77.4 ± 2.8 a | 3.0 ± 0.2 a | 9.8 ± 0.4 a |
Ph 15-15-15 | 112.2 ± 9.4 a | 59.6 ± 6.1 a | 85.7 ± 5.1 a | 56.7 ± 7.4 a | 70.9 ± 7.5 a | 77.7 ± 3.7 a | 2.8 ± 0.2 a | 9.7 ± 0.3 a |
SP1 | SP2 | SP3 | SP4 | |||||
---|---|---|---|---|---|---|---|---|
N | 11,893 | 10,098 | 11,210 | 8142 | ||||
Parameter | Mean ± SEM | Range | Mean ± SEM | Range | Mean ± SEM | Range | Mean ± SEM | Range |
VCL (µm/s) | 72.7 ± 0.1 | 0.0–120.0 | 109.1 ± 0.1 | 71.6–181.9 | 120.1 ± 0.2 | 87.2–213.9 | 158.3 ± 0.3 | 83.1–372.2 |
VSL (µm/s) | 45.9 ± 0.1 | 0.0–84.6 | 64.7 ± 0.2 | 0.9-105.6 | 94.5 ± 0.2 | 43.4–199.0 | 47.2 ± 0.3 | 0.6–221.2 |
VAP (µm/s) | 59.9 ± 0.1 | 0.0–112.9 | 85.1 ± 0.1 | 34.4–129.3 | 112.7 ± 0.2 | 83.9–224.8 | 109.5 ± 0.2 | 24.1-282.5 |
LIN (%) | 63.2 ± 0.2 | 0.0–100.0 | 60.7 ± 0.2 | 0.8–97.7 | 79.4 ± 0.1 | 33.8–99.3 | 30.0 ± 0.2 | 0.4–84.4 |
STR (%) | 72.1 ± 0.2 | 0.0–100.0 | 76.6 ± 0.2 | 1.4–99.7 | 84.3 ± 0.1 | 35.4–100.0 | 43.7 ± 0.2 | 0.5–98.3 |
WOB (%) | 80.6 ± 0.1 | 0.0–100.0 | 78.6 ± 0.1 | 28.1–100.0 | 93.8 ± 0.1 | 71.2–100.0 | 69.7 ± 0.2 | 17.6–100.0 |
ALH (µm) | 2.2 ± 0.1 | 0.0–5.9 | 3.5 ± 0.1 | 0.9–6.6 | 2.5 ± 0.1 | 0.4–5.30 | 5.7 ± 0.1 | 2.0–16.9 |
BCF (Hz) | 7.7 ± 0.1 | 0.0–21.0 | 11.0 ± 0.1 | 3.6–22.0 | 7.9 ± 0.1 | 0.0–20.0 | 8.4 ± 0.1 | 0.0–22.0 |
Patterns | % DFI | Mean DFI | % HDS |
---|---|---|---|
Control | 15.4 ± 3.0 | 322.9 ± 30.1 | 21.1 ± 5.1 |
Photo 1 | 15.4 ± 0.8 | 335.5 ± 33.3 | 22.8 ± 8.9 |
Photo 2 | 15.4 ± 1.2 | 332.1 ± 19.5 | 22.8 ± 7.6 |
Photo 3 | 15.9 ± 2.9 | 327.5 ± 25.4 | 22.2 ± 5.1 |
Photo 4 | 14.2 ± 1.4 | 319.0 ± 21.3 | 18.1 ± 5.6 |
Photo 5 | 15.8 ± 1.4 | 326.9 ± 29.9 | 19.7 ± 8.9 |
Photo 10 | 14.6 ± 0.9 | 319.7 ± 25.4 | 19.3 ± 8.5 |
Photo 1-1-1 | 17.0 ± 1.4 | 326.0 ± 34.8 | 25.3 ± 8.1 |
Photo 2-1-2 | 15.6 ± 0.7 | 319.7 ± 29.0 | 21.1 ± 5.8 |
Photo 2-2-2 | 17.1 ± 1.7 | 332.6 ± 30.1 | 25.9 ± 6.1 |
Photo 3-1-3 | 16.4 ± 2.9 | 351.7 ± 46.2 | 22.7 ± 8.9 |
Photo 3-3-3 | 15.8 ± 2.9 | 330.4 ± 30.2 | 21.8 ± 3.1 |
Photo 4-1-4 | 14.3 ± 1.0 | 332.5 ± 26.4 | 18.7 ± 8.3 |
Photo 4-4-4 | 16.8 ± 2.9 | 357.7 ± 44.1 | 32.3 ± 7.2 |
Photo 5-1-5 | 17.5 ± 3.9 | 323.7 ± 28.1 | 28.2 ± 12.2 |
Photo 5-5-5 | 17.5 ± 2.7 | 350.0 ± 37.5 | 31.6 ± 6.0 |
Photo 10-5-10 | 18.4 ± 3.8 | 341.4 ± 27.0 | 31.9 ± 7.0 |
Photo 10-10-10 | 18.4 ± 3.3 | 324.6 ± 27.4 | 31.4 ± 10.4 |
Photo 15-5-15 | 19.3 ± 4.5 | 313.7 ± 24.3 | 29.7 ± 9.2 |
Photo 15-15-15 | 18.3 ± 4.3 | 314.5 ± 19.3 | 25.1 ± 9.6 |
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Catalán, J.; Papas, M.; Gacem, S.; Mateo-Otero, Y.; Rodríguez-Gil, J.E.; Miró, J.; Yeste, M. Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure. Biology 2020, 9, 254. https://doi.org/10.3390/biology9090254
Catalán J, Papas M, Gacem S, Mateo-Otero Y, Rodríguez-Gil JE, Miró J, Yeste M. Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure. Biology. 2020; 9(9):254. https://doi.org/10.3390/biology9090254
Chicago/Turabian StyleCatalán, Jaime, Marion Papas, Sabrina Gacem, Yentel Mateo-Otero, Joan E. Rodríguez-Gil, Jordi Miró, and Marc Yeste. 2020. "Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure" Biology 9, no. 9: 254. https://doi.org/10.3390/biology9090254