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Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression

1
School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea
2
Korea Basic Science Institute, Gwangju Center at Chonnam National University, Gwangju 61886, Korea
3
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
*
Authors to whom correspondence should be addressed.
Both authors contributed equally.
Biology 2020, 9(8), 212; https://doi.org/10.3390/biology9080212
Received: 1 July 2020 / Revised: 27 July 2020 / Accepted: 4 August 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Molecular Mechanism of Histone Modification and Gene Regulation)
Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In this study, we present the anti-osteoporotic activity of A. distichum extract by inhibiting osteoclast formation. First, we show that the methanolic extract of the leaves of A. distichum, but not extracts of the branches or fruits, significantly inhibits receptor activator of the NF-κB ligand (RANKL)-induced osteoclast differentiation. Second, our transcriptome analysis revealed that the leaf extract (LE) blocks sets of RANKL-mediated osteoclast-related genes. Third, the LE attenuates the phosphorylation of extracellular signal-related kinase. Finally, treatment with the LE effectively prevents postmenopausal bone loss in ovariectomized mice and glucocorticoid-induced osteoporosis in zebrafish. Our findings show that the extract of A. distichum efficiently suppressed osteoclastogenesis by regulating osteoclast-related genes, thus offering a novel therapeutic strategy for osteoporosis. View Full-Text
Keywords: Abeliophyllum distichum Nakai; osteoclast; NFATc1; osteoporosis Abeliophyllum distichum Nakai; osteoclast; NFATc1; osteoporosis
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MDPI and ACS Style

Lee, K.; Jang, Y.-J.; Lee, H.; Kim, E.; Kim, Y.; Yoo, T.-K.; Hyun, T.K.; Park, J.-I.; Yi, S.-J.; Kim, K. Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression. Biology 2020, 9, 212. https://doi.org/10.3390/biology9080212

AMA Style

Lee K, Jang Y-J, Lee H, Kim E, Kim Y, Yoo T-K, Hyun TK, Park J-I, Yi S-J, Kim K. Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression. Biology. 2020; 9(8):212. https://doi.org/10.3390/biology9080212

Chicago/Turabian Style

Lee, Kyubin, You-Jee Jang, Hyerim Lee, Eunbin Kim, Yeojin Kim, Tong-Kewn Yoo, Tae K. Hyun, Jae-Il Park, Sun-Ju Yi, and Kyunghwan Kim. 2020. "Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression" Biology 9, no. 8: 212. https://doi.org/10.3390/biology9080212

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