Next Article in Journal
Oxidative Stress in Male Infertility: Causes, Effects in Assisted Reproductive Techniques, and Protective Support of Antioxidants
Previous Article in Journal
Polymorphisms in Interleukin 13 Signaling and Interacting Genes Predict Advanced Fibrosis and Hepatocellular Carcinoma Development in Non-Alcoholic Steatohepatitis
Open AccessArticle

Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

1
Faculty of Biology, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, Ward 4, District 5, Ho Chi Minh 72711, Vietnam
2
Faculty of Biotechnology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh 72820, Vietnam
3
Biotechnology Center of Ho Chi Minh City, 2374 Highway 1, Quarter 2, Ward Trung My Tay, District 12, Ho Chi Minh 71507, Vietnam
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the works.
Biology 2020, 9(4), 76; https://doi.org/10.3390/biology9040076
Received: 15 March 2020 / Revised: 9 April 2020 / Accepted: 9 April 2020 / Published: 10 April 2020
Dendrobium has been widely used not only as ornamental plants but also as food and medicines. The identification and evaluation of the genetic diversity of Dendrobium species support the conservation of genetic resources of endemic Dendrobium species. Uniquely identifying Dendrobium species used as medicines helps avoid misuse of medicinal herbs. However, it is challenging to identify Dendrobium species morphologically during their immature stage. Based on the DNA barcoding method, it is now possible to efficiently identify species in a shorter time. In this study, the genetic diversity of 76 Dendrobium samples from Southern Vietnam was investigated based on the ITS (Internal transcribed spacer), ITS2, matK (Maturase_K), rbcL (ribulose-bisphosphate carboxylase large subunit) and trnH-psbA (the internal space of the gene coding histidine transfer RNA (trnH) and gene coding protein D1, a polypeptide of the photosystem I reaction center (psaB)) regions. The ITS region was found to have the best identification potential. Nineteen out of 24 Dendrobium species were identified based on phylogenetic tree and Indel information of this region. Among these, seven identified species were used as medicinal herbs. The results of this research contributed to the conservation, propagation, and hybridization of indigenous Dendrobium species in Southern Vietnam. View Full-Text
Keywords: Dendrobium; ITS; ITS2; matK; rbcL; trnH-psbA; southern Vietnam; molecular identification; genetic diversity; DNA barcoding Dendrobium; ITS; ITS2; matK; rbcL; trnH-psbA; southern Vietnam; molecular identification; genetic diversity; DNA barcoding
Show Figures

Figure 1

MDPI and ACS Style

Nguyen, N.-H.; Vu, H.-T.; Le, N.-D.; Nguyen, T.-D.; Duong, H.-X.; Tran, H.-D. Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam. Biology 2020, 9, 76.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop