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Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of Thalassiosira Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series

Graduate School of Oceanography, University of Rhode Island, South Ferry Road, Narragansett, RI 02882, USA
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Biology 2020, 9(1), 19; https://doi.org/10.3390/biology9010019
Received: 10 December 2019 / Revised: 6 January 2020 / Accepted: 7 January 2020 / Published: 16 January 2020
(This article belongs to the Special Issue The Molecular Life of Diatoms: From Genes to Ecosystems)
Diatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus Thalassiosira were examined at the site of the Narragansett Bay (NBay) Long-Term Plankton Time Series using a combination of light microscopy (LM), high-throughput sequencing (HTS) of the 18S rDNA V4 region and historical records. Thalassiosira species were identified over 6 years using a combination of LM and DNA sequences. Sixteen Thalassiosira taxa were identified using HTS: nine were newly identified in NBay. Several newly identified species have small cell diameters and are difficult to identify using LM. However, they appeared frequently and thus may play a significant ecological role in NBay, particularly since their realized niches suggest they are eurythermal and able to tolerate the >25 °C temperature range of NBay. Four distinct species assemblages that grouped by season were best explained by surface water temperature. When compared to historical records, we found that the cold-water species Thalassiosira nordenskioeldii has decreased in persistence over time, suggesting that increasing surface water temperature has influenced the ecology of phytoplankton in NBay. View Full-Text
Keywords: community composition; diatom; diversity; high throughput sequencing; molecular ecology; phytoplankton; metabarcoding community composition; diatom; diversity; high throughput sequencing; molecular ecology; phytoplankton; metabarcoding
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Rynearson, T.A.; Flickinger, S.A.; Fontaine, D.N. Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of Thalassiosira Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series. Biology 2020, 9, 19.

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