Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes
AbstractExosomes are small extracellular vesicles that have emerged as an important tool for intercellular communication. In the central nervous system, exosomes can mediate glia and neuronal communication. Once released from the donor cell, exosomes can act as discrete vesicles and travel to distant and proximal recipient cells to alter cellular function. Microglia cells secrete exosomes due to stress stimuli of alcohol abuse. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of exosomes derived from microglia cell line BV-2. The BV-2 cells were cultured in exosome-free media and were either mock treated (control) or treated with 50 mM or 100 mM of alcohol for 48 and 72 h. Our results demonstrated that alcohol significantly impacted BV-2 cell morphology, viability, and protein content. Most importantly, our studies revealed that exosome biogenesis and composition was affected by alcohol treatment. View Full-Text
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Crenshaw, B.J.; Kumar, S.; Bell, C.R.; Jones, L.B.; Williams, S.D.; Saldanha, S.N.; Joshi, S.; Sahu, R.; Sims, B.; Matthews, Q.L. Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes. Biology 2019, 8, 25.
Crenshaw BJ, Kumar S, Bell CR, Jones LB, Williams SD, Saldanha SN, Joshi S, Sahu R, Sims B, Matthews QL. Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes. Biology. 2019; 8(2):25.Chicago/Turabian Style
Crenshaw, Brennetta J.; Kumar, Sanjay; Bell, Courtnee’ R.; Jones, Leandra B.; Williams, Sparkle D.; Saldanha, Sabita N.; Joshi, Sameer; Sahu, Rajnish; Sims, Brian; Matthews, Qiana L. 2019. "Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes." Biology 8, no. 2: 25.
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