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Biology 2019, 8(2), 21; https://doi.org/10.3390/biology8020021

Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

1
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan
2
Kobayashi Branch: Miyazaki Prefectural Fisheries Research Institute, Kobayashi, Miyazaki 886-0005, Japan
3
School of Marine Biosciences, Kitasato University, Minami, Sagamihara, Kanagawa 252-0313, Japan
*
Author to whom correspondence should be addressed.
Received: 7 February 2019 / Revised: 25 March 2019 / Accepted: 25 March 2019 / Published: 29 March 2019
(This article belongs to the Special Issue Fish Metabolic Physiology in Response to Stress)
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Abstract

Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (Oncorhynchus masou, known in Japan as ‘yamame’), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adipose fin were identified by comparing the HT and NT groups. Functional analyses were then performed to identify associated gene ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The HT group showed a high expression of heat shock protein 70 (HSP70) gene and enriched gene expression in the extracellular matrix (ECM), cell junction, and adhesion pathways in gill tissues compared to the NT group. The HT group also exhibited highly expressed genes in glycolysis and showed lower expression of the genes in the p53 signaling pathway in adipose fin tissues. Taken together, the difference of expression of some genes in the normal condition may be responsible for the difference in heat tolerance between the HT and NT yamame in the heat stress condition. View Full-Text
Keywords: high-temperature tolerance; yamame; selective breeding; differentially expressed genes; heat shock protein high-temperature tolerance; yamame; selective breeding; differentially expressed genes; heat shock protein
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Kraitavin, W.; Yoshitake, K.; Igarashi, Y.; Mitsuyama, S.; Kinoshita, S.; Kambayashi, D.; Watabe, S.; Asakawa, S. Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress. Biology 2019, 8, 21.

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