Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

Iwano, Hidetomo; Inoue, Yusuke; Takasago, Takuji; Kobayashi, Hironori; Furusawa, Takaaki; Taniguchi, Kotomi; Fujiki, Jumpei; Yokota, Hiroshi; Usui, Masaru; Tanji, Yasunori; Hagiwara, Katsuro; Higuchi, Hidetoshi; Tamura, Yutaka

doi:10.3390/biology7010008

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Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

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Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

Laboratory of Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

Department of Bioengineering, Tokyo Institute of Technology, Yokohama 226-8502, Japan

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Laboratory of Veterinary Virology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

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Laboratory of Veterinary Hygiene, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

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Center for Veterinary Drug Vevelopment, Rakuno Gakuen University, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

Author to whom correspondence should be addressed.

Biology 2018, 7(1), 8; https://doi.org/10.3390/biology7010008

Received: 22 November 2017 / Revised: 4 January 2018 / Accepted: 5 January 2018 / Published: 9 January 2018

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Abstract

Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It is the most costly disease in the dairy industry because of the high use of antibiotics. Staphylococcus aureus is one of the major causative agents of bovine mastitis and antimicrobial resistance. Therefore, new strategies to control bacterial infection are required in the dairy industry. One potential strategy is bacteriophage (phage) therapy. In the present study, we examined the host range of previously isolated S. aureus phages ΦSA012 and ΦSA039 against S. aureus strains isolated from mastitic cows. These phages could kill all S. aureus (93 strains from 40 genotypes) and methicillin-resistant S. aureus (six strains from six genotypes) strains tested. Using a mouse mastitis model, we demonstrated that ΦSA012 reduced proliferation of S. aureus and inflammation in the mammary gland. Furthermore, intravenous or intraperitoneal phage administration reduced proliferation of S. aureus in the mammary glands. These results suggest that broad host range phages ΦSA012 is potential antibacterial agents for dairy production medicine. View Full-Text

Keywords: bacteriophage; mastitis; Staphylococcus aureus

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Iwano, H.; Inoue, Y.; Takasago, T.; Kobayashi, H.; Furusawa, T.; Taniguchi, K.; Fujiki, J.; Yokota, H.; Usui, M.; Tanji, Y.; Hagiwara, K.; Higuchi, H.; Tamura, Y. Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model. Biology 2018, 7, 8. https://doi.org/10.3390/biology7010008

AMA Style

Iwano H, Inoue Y, Takasago T, Kobayashi H, Furusawa T, Taniguchi K, Fujiki J, Yokota H, Usui M, Tanji Y, Hagiwara K, Higuchi H, Tamura Y. Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model. Biology. 2018; 7(1):8. https://doi.org/10.3390/biology7010008

Chicago/Turabian Style

Iwano, Hidetomo, Yusuke Inoue, Takuji Takasago, Hironori Kobayashi, Takaaki Furusawa, Kotomi Taniguchi, Jumpei Fujiki, Hiroshi Yokota, Masaru Usui, Yasunori Tanji, Katsuro Hagiwara, Hidetoshi Higuchi, and Yutaka Tamura. 2018. "Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model" Biology 7, no. 1: 8. https://doi.org/10.3390/biology7010008

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Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model

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