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Ecological Restoration of Limestone Tailings in Arid Regions: A Synergistic Substrate–Plant Approach
by
Wei Hou
1,
Dunzhu Pubu
1,*,
Duoji Bianba
1,
Zeng Dan
1,
Zengtao Jin
1,
Qunzong Gama
1,
Jingjing Hu
1,
Yang Li
2 and
Zhuxin Mao
2 1
Forest Inventory and Planning Institute of Tibet Autonomous Region, Lhasa 850000, China
2
Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, China
*
Author to whom correspondence should be addressed.
Biology 2026, 15(1), 82; https://doi.org/10.3390/biology15010082 (registering DOI)
Submission received: 3 December 2025
/
Revised: 21 December 2025
/
Accepted: 26 December 2025
/
Published: 31 December 2025
(This article belongs to the Section Ecology)
Simple Summary
Mining limestone in dry areas leaves behind piles of leftover rock and soil, called tailings, which damage the environment and need to be restored. We aimed to find the best way to help plants grow on these difficult sites in the arid regions of Northern China. We tested different mixes of natural soil with tailings and different water and nitrogen levels to see what would help native grass species grow best. We found that a mix of two parts soil to one part tailings, kept moderately moist, yielded the best results. Nutrient dynamics ultimately governed biomass accumulation, accounting for 57.8–84.2% of the biomass variation. Among the plants tested, Pennisetum centrasiaticum and Setaria viridis demonstrated the best overall performance, based on their comprehensive evaluation scores. This study shows that the key to restoring these areas is using the right soil mix, managing water carefully, and planting a smart combination of deep- and shallow-rooted grasses to use all available resources. These findings provide a practical, science-based guide for repairing damaged mining landscapes, which will help return life to these barren areas and improve the environment for local communities.
Abstract
In arid regions, the ecological restoration of limestone tailings requires sustainable strategies, yet the synergistic effects of substrate optimization and native plant selection remain poorly understood. In this study, we systematically evaluated substrate amendments and native species for rehabilitating limestone tailings in Northern China’s arid zone using a controlled pot experiment. An orthogonal L9(34) experimental design was employed to test three factors: the soil-to-tailings ratio (1:2, 1:1, and 2:1), moisture level (30%, 45%, and 60% of field capacity), and nitrogen addition (0, 5, and 10 g N m−2). Five native grass species (Pennisetum centrasiaticum, Setaria viridis, Leymus chinensis, Achnatherum splendens, and Eleusine indica) were grown under these treatment conditions, and plant biomass and key soil nutrient variables were measured. Stepwise regression, structural equation modeling, and principal component analysis were applied to assess plant growth responses and soil nutrient dynamics. The results indicated that a 2:1 soil-to-tailings substrate maintained at 60% moisture content maximized biomass production across all species. Soil total potassium consistently correlated positively with biomass (Standardized β: 0.397–0.603), whereas available potassium showed a negative relationship (Standardized β: −0.825–−0.391). Nutrient dynamics ultimately governed biomass accumulation, accounting for 57.8–84.2% of the biomass variation. P. centrasiaticum ranked as the most effective species, followed by S. viridis, L. chinensis, A. splendens, and E. indica. We concluded that successful restoration under these experimental conditions hinged on key factors: using a 2:1 soil-to-tailings substrate, maintaining 60% soil moisture, and strategically combining deep-rooted P. centrasiaticum with shallow-rooted S. viridis to exploit complementary resource use. This work provides fundamental data and a conceptual framework for rehabilitating arid limestone tailings in similar ecological settings, based on controlled experimental evidence.
