Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Library Preparation and RNA-Seq
2.3. Data Analysis
2.4. GO and KEGG Enrichment Analysis of DEGs
2.5. Quantitative Real-Time PCR Validation
3. Result
3.1. Information Obtained from the Sequencing Data
3.2. Dynamic Expression of the Transcriptome under Low Salinity and Pathogenic Challenge
3.3. Expression Pattern Clustering of DEGs
3.4. Screening of Reverse DEGs after Exposure to the Two Kinds of Stress
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sun, D.; Lv, J.; Li, Y.; Wu, J.; Liu, P.; Gao, B. Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus. Biology 2023, 12, 1518. https://doi.org/10.3390/biology12121518
Sun D, Lv J, Li Y, Wu J, Liu P, Gao B. Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus. Biology. 2023; 12(12):1518. https://doi.org/10.3390/biology12121518
Chicago/Turabian StyleSun, Dongfang, Jianjian Lv, Yukun Li, Jie Wu, Ping Liu, and Baoquan Gao. 2023. "Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus" Biology 12, no. 12: 1518. https://doi.org/10.3390/biology12121518
APA StyleSun, D., Lv, J., Li, Y., Wu, J., Liu, P., & Gao, B. (2023). Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus. Biology, 12(12), 1518. https://doi.org/10.3390/biology12121518