Integrated Analysis of the Intestinal Microbiota and Transcriptome of Fenneropenaeus chinensis Response to Low-Salinity Stress
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Shrimp Rearing and Sample Collection
2.2. Microbiota Analysis Using 16S rDNA Genes Sequencing
2.3. Gene Expression Analysis Using RNA Sequencing
2.4. Verification of Real-Time Quantitative PCR (qPCR)
2.5. Correlations between ASVs and DEGs
3. Results
3.1. Intestinal Microbiota Community Analysis
3.2. Intestinal Transcriptome Analysis
3.3. Correlation between Intestinal Microbiota and Immune-Related DEGs
4. Discussion
4.1. Intestinal Microbial Community Changed under Low-Salinity Stress
4.2. Participation of Immune-Related Genes in Response to Low-Salinity Stress
4.3. Relationship between Intestinal Microbial Community and Expression of Immune-Related Genes
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tian, C.; Wang, Q.; Wang, J.; Li, J.; Guan, C.; He, Y.; Gao, H. Integrated Analysis of the Intestinal Microbiota and Transcriptome of Fenneropenaeus chinensis Response to Low-Salinity Stress. Biology 2023, 12, 1502. https://doi.org/10.3390/biology12121502
Tian C, Wang Q, Wang J, Li J, Guan C, He Y, Gao H. Integrated Analysis of the Intestinal Microbiota and Transcriptome of Fenneropenaeus chinensis Response to Low-Salinity Stress. Biology. 2023; 12(12):1502. https://doi.org/10.3390/biology12121502
Chicago/Turabian StyleTian, Caijuan, Qiong Wang, Jiajia Wang, Jitao Li, Chenhui Guan, Yuying He, and Huan Gao. 2023. "Integrated Analysis of the Intestinal Microbiota and Transcriptome of Fenneropenaeus chinensis Response to Low-Salinity Stress" Biology 12, no. 12: 1502. https://doi.org/10.3390/biology12121502