Challenges and Approaches of Culturing the Unculturable Archaea
Abstract
:Simple Summary
Abstract
1. Introduction
2. Significance of Archaea and Archaeal Culturomics
3. Challenges in Archaeal Culturomics
3.1. Environmental Conditions/Abiotic Conditions
3.2. Suitable Nutritional Requirements (Concentration of Substrates)
3.3. Consortium and Co-Culturing
3.4. Inappropriate Incubation Time
3.5. Low Abundance, Presence of Persisters, and Dead Cells
3.6. Small Genome Size of Archaea (Symbiotic Association)
3.7. Incorrect Genome Annotation
4. Current Status of Culturomics for Archaea
5. Culturomics Strategies for Archaea
5.1. Simulation Strategies of Natural Habitat (High-Throughput Culturing)
5.2. Co-Culturing
5.3. Single-Cell Sorting
5.4. Culture Media for Halophilic Archaea
5.5. Culture Media for Methanogenic Archaea
5.6. Culture Media for Thermophilic and Hyperthermophilic Archaea
5.7. Culture Media for Mesophilic Archaea
5.8. Culture Media for Acidophiles Archaea
5.9. Proposed Basal Media for Archaeal Cultivation
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rafiq, M.; Hassan, N.; Rehman, M.; Hayat, M.; Nadeem, G.; Hassan, F.; Iqbal, N.; Ali, H.; Zada, S.; Kang, Y.; et al. Challenges and Approaches of Culturing the Unculturable Archaea. Biology 2023, 12, 1499. https://doi.org/10.3390/biology12121499
Rafiq M, Hassan N, Rehman M, Hayat M, Nadeem G, Hassan F, Iqbal N, Ali H, Zada S, Kang Y, et al. Challenges and Approaches of Culturing the Unculturable Archaea. Biology. 2023; 12(12):1499. https://doi.org/10.3390/biology12121499
Chicago/Turabian StyleRafiq, Muhammad, Noor Hassan, Maliha Rehman, Muhammad Hayat, Gullasht Nadeem, Farwa Hassan, Naveed Iqbal, Hazrat Ali, Sahib Zada, Yingqian Kang, and et al. 2023. "Challenges and Approaches of Culturing the Unculturable Archaea" Biology 12, no. 12: 1499. https://doi.org/10.3390/biology12121499