Evolutionary Plasticity in Insular Lizard, Adapting over Reproduction, Metabolism, and Color Variation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Study Area and Samples
2.2. RNA Isolation
2.3. Transcriptome Analyses
2.4. Differential Gene Expression between Populations
2.5. Verification of Differentially Expressed Genes by Real-Time PCR
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Name | Sequence Primer 5‘-3‘ | Amplicon Size (bp) |
---|---|---|---|
GnRH-R2 | GNRH_1524F | GTCAATGGAAACCCAACGGC | 166 |
GNRH_1689R | ATGTTGAAGCAGGCGGAAGA | ||
Actin | ACTINA_PS_R | GATCTGGCACCACACCTTCT | 104 |
ACTINA_PSC_F | TCTTTTCTCTGTTGGCTTTGG |
Transcripts FPKM > 1 | 373.072 |
%GC | 45.04 |
Average contig length | 821.89 |
N50 (bp) | 1577 |
Assembled bases (Mbp) | 307 |
Gene | Description |
---|---|
GATA3 | Trans-acting T-cell-specific transcription factor GATA-3 |
PAD-DJ-1 | Parkinsonism associated deglycase |
PITX3 | Pituitary homeobox 2-like |
HS71 | Heat shock cognate 71 kDa protein-like isoform X1 |
tilB | Leucine rich repeat containing 6 |
GnRH-R | Type 3/II gonadotropin-releasing hormone receptor |
TRIP4 | Thyroid hormone receptor interactor 4 |
STAT3 | Signal transducer and activator of transcription |
EF2 | Eukaryotic elongation factor 2 |
RWD | RWD domain containing 1 |
PG4 | platelet glycoprotein 4 isoform X2 |
POU | POU domain protein |
LEB-1 | Isoform 6 of lymphoid enhancer-binding factor 1 |
Neurabin-2 | Neurabin-2 |
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Fulgione, D.; Maselli, V.; Rivieccio, E.; Aceto, S.; Salvemini, M.; Buglione, M. Evolutionary Plasticity in Insular Lizard, Adapting over Reproduction, Metabolism, and Color Variation. Biology 2023, 12, 1478. https://doi.org/10.3390/biology12121478
Fulgione D, Maselli V, Rivieccio E, Aceto S, Salvemini M, Buglione M. Evolutionary Plasticity in Insular Lizard, Adapting over Reproduction, Metabolism, and Color Variation. Biology. 2023; 12(12):1478. https://doi.org/10.3390/biology12121478
Chicago/Turabian StyleFulgione, Domenico, Valeria Maselli, Eleonora Rivieccio, Serena Aceto, Marco Salvemini, and Maria Buglione. 2023. "Evolutionary Plasticity in Insular Lizard, Adapting over Reproduction, Metabolism, and Color Variation" Biology 12, no. 12: 1478. https://doi.org/10.3390/biology12121478
APA StyleFulgione, D., Maselli, V., Rivieccio, E., Aceto, S., Salvemini, M., & Buglione, M. (2023). Evolutionary Plasticity in Insular Lizard, Adapting over Reproduction, Metabolism, and Color Variation. Biology, 12(12), 1478. https://doi.org/10.3390/biology12121478