IDH1 Promotes Foam Cell Formation by Aggravating Macrophage Ferroptosis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Data Mining from the Gene Expression Omnibus (GEO) Database
2.2. Analysis of Functional Enrichment
2.3. FerrDb Database
2.4. Cells and Reagents
2.5. Treatment and Transfection of Cells
2.6. Analysis of Cell Viability
2.7. Western Blotting Analysis
2.8. Reverse Transcription-Quantitative PCR (RT-qPCR)
2.9. Measurement of Intracellular GSH Level
2.10. Iron Assay
2.11. Lipid Peroxidation Assay
2.12. Annexin V-APC/7-AAD Staining and Flow Cytometry
2.13. Oil Red O Staining
2.14. LDH Release Assay
2.15. α-KG Assay
2.16. NADPH Assay
2.17. JASPAR Analysis
2.18. Chromatin Immunoprecipitation (ChIP) Assay
2.19. Statistical Analysis
3. Results
3.1. Identification and Enrichment Analysis of DEGs in Foam Cells
3.2. Identification and Enrichment Analysis of Target DEGs
3.3. Expression of IDH1 in ox-LDL-Induced Foam Cells
3.4. Inhibition of IDH1 Can Reduce the Production of ox-LDL-Induced Foam Cells
3.5. Inhibition of IDH1 Ameliorates ox-LDL-Induced Ferroptosis
3.6. Inhibition of IDH1 Suppresses α-KG and NADPH Levels
3.7. Inhibition of IDH1 Ameliorates Macrophage Damage and Apoptosis Induced by ox-LDL
3.8. IDH1 May Regulate ox-LDL-Induced Macrophage Ferroptosis via NRF2
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, B.; Wang, C.; Lu, P.; Ji, Y.; Wang, X.; Liu, C.; Lu, X.; Xu, X.; Wang, X. IDH1 Promotes Foam Cell Formation by Aggravating Macrophage Ferroptosis. Biology 2022, 11, 1392. https://doi.org/10.3390/biology11101392
Li B, Wang C, Lu P, Ji Y, Wang X, Liu C, Lu X, Xu X, Wang X. IDH1 Promotes Foam Cell Formation by Aggravating Macrophage Ferroptosis. Biology. 2022; 11(10):1392. https://doi.org/10.3390/biology11101392
Chicago/Turabian StyleLi, Ben, Chufan Wang, Peng Lu, Yumeng Ji, Xufeng Wang, Chaoyang Liu, Xiaohu Lu, Xiaohan Xu, and Xiaowei Wang. 2022. "IDH1 Promotes Foam Cell Formation by Aggravating Macrophage Ferroptosis" Biology 11, no. 10: 1392. https://doi.org/10.3390/biology11101392