Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. TILs Scoring
2.3. Transient Knockdown
sense (5′-3′) | antisense (5′-3′) |
CCGUAUUCCCUGUAUGUCUTT | AGACAUACAGGGAAUACGGT |
GCAAAGAUGCACCAUCCAATT | UUGGAUGGUGCAUCUUUGCTT |
GCAACAUUCAAGGGAUUGATT | UCAAUCCCUUGAAUGUUGCTT |
2.4. mIF and IHC
2.5. Dataset Analysis
2.6. Statistical Analysis
3. Results
3.1. Cohort Description and Image Cytometry
3.2. Increased VISTA and IBA-1 Expression in the TME
3.3. Phenotypes of the CD8+ T-Cells
3.4. Validation of mIF Using IHC and RNA Expression Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Croft, P.K.-d.; Chittoory, H.; Nguyen, T.H.; Saunus, J.M.; Kim, W.G.; McCart Reed, A.E.; Lim, M.; De Luca, X.M.; Ferguson, K.; Niland, C.; et al. Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases. Biology 2021, 10, 425. https://doi.org/10.3390/biology10050425
Croft PK-d, Chittoory H, Nguyen TH, Saunus JM, Kim WG, McCart Reed AE, Lim M, De Luca XM, Ferguson K, Niland C, et al. Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases. Biology. 2021; 10(5):425. https://doi.org/10.3390/biology10050425
Chicago/Turabian StyleCroft, Priyakshi Kalita-de, Haarika Chittoory, Tam H. Nguyen, Jodi M. Saunus, Woo Gyeong Kim, Amy E. McCart Reed, Malcolm Lim, Xavier M. De Luca, Kaltin Ferguson, Colleen Niland, and et al. 2021. "Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases" Biology 10, no. 5: 425. https://doi.org/10.3390/biology10050425
APA StyleCroft, P. K. -d., Chittoory, H., Nguyen, T. H., Saunus, J. M., Kim, W. G., McCart Reed, A. E., Lim, M., De Luca, X. M., Ferguson, K., Niland, C., Mazzieri, R., Dolcetti, R., Simpson, P. T., & Lakhani, S. R. (2021). Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases. Biology, 10(5), 425. https://doi.org/10.3390/biology10050425