Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Sample Collection
2.2. The Isolation and Induced Differentiation of Yak Preadipocytes
2.3. Cell Culture and Transfection
2.4. Cell Induced Differentiation and Oil Red O Staining
2.5. Triglyceride Assay
2.6. CCK-8 Proliferation Assay
2.7. EdU Proliferation Assay
2.8. qRT-PCR Analysis
2.9. Luciferase Reporter Assay
2.10. Western Blot Analysis
2.11. Statistical Analysis
3. Results
3.1. Expression of Bta-miR-2400 in the Yak Tissues and Adipocytes
3.2. Bta-miR-2400 Promotes the Proliferation of Yak Preadipocytes
3.3. Role of Bta-miR-2400 during Yak Preadipocytes Lipogenic Differentiation
3.4. Bta-miR-2400 Directly Targets the 3′UTR of SUMO1
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequence (5′ to 3′) | Tm/°C | Product Size/bp | Accession Numbers |
---|---|---|---|---|
SCD | F:CTGGTGTCCTGTTGTTGTGC R:GGTCTTGTCATAAGGGCGGT | 60.0 | 179 | XM_005892055.2 |
PPAR γ | F:CATTTCCACTCCGCACTA R:GGGATACAGGCTCCACTT | 60.3 | 122 | XM_005902846.1 |
C/EBPα | F:GGCATCTGCGAACACGAGA R:AGGAACTCGTCGTTGAAGGC | 60.3 | 212 | XM_005893918.2 |
FABP4 | F:CATTAAATCCGAAAGCA R:CTCATAAACTCTGGTGGC | 60.3 | 242 | XM_014478668.1 |
FAS | F:GCAAAGTGGTCATTCAGGTACG R:CCCAGTGATGATGTAGCTCTTG | 64.5 | 125 | NM_001012669.1 |
ACC | F:GAGACAAACAGGGACCATT R:AGGGACTGCCGAAACAT | 61.4 | 142 | AJ132890.1 |
CPT1 | F:TCGACCCAAACAAGTACCCC R:CGCTGGGCATTTGTCTCTGA | 60.0 | 157 | NM_001034349.2 |
DGAT1 | F:GAGACAAACAGGGACCATT R: AGGGACTGCCGAAACAT | 64.5 | 92 | JF913457.1 |
SUMO1 | F:CATTGGACAGGATAGCA R:CTCCATTCCCAGTTCTT | 59.0 | 172 | NM_001035458.1 |
ELOVL1 | F:AGAAAGACGGACAGGTGAC R:GCAGGAAGTAGTATTGGGAG | 61.4 | 273 | XM_010803685.3 |
bta-miR-2400 | F:CCAGCACAGGCAGCTCGGACTGA | 60.3 | _ | |
SREBPF1 | F:GCTGACCGACATAGAAGACA R:CTCATCGTGGAAGGAGGTGG | 61.0 | 165 | NM_001113302.1 |
PCNA | F:TCCGAGGGCTTCGACACTTA R:GTGCCAACGTGTCTGCGTTA | 63.3 | 142 | XM_005906528.2 |
CDK2 | F:AGGTGGTGACTCTGTGGTA R:GCTCCGTCCATCTTCAT | 59.0 | 299 | XM_005905862.2 |
CDK4 | F:TTGGTGTCGGTGCCTAT R:TCCAGACGCCGCAGTAA | 57.0 | 157 | XM_005901664.1 |
U6 | F:GGAACGATACAGAGAAGATTAGC R:TGGAACGCTTCACGAATTTGCG | 60.3 | _ | |
β-Actin | F:GCAGGTCATCACCATCGG R:CCGTGTTGGCGTAGAGGT | 60.3 | 158 | XM_005887322.2 |
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Zhang, Y.; Ma, L.; Gu, Y.; Chang, Y.; Liang, C.; Guo, X.; Bao, P.; Chu, M.; Ding, X.; Yan, P. Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation. Biology 2021, 10, 949. https://doi.org/10.3390/biology10100949
Zhang Y, Ma L, Gu Y, Chang Y, Liang C, Guo X, Bao P, Chu M, Ding X, Yan P. Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation. Biology. 2021; 10(10):949. https://doi.org/10.3390/biology10100949
Chicago/Turabian StyleZhang, Yongfeng, Lanhua Ma, Yarong Gu, Yongfang Chang, Chunnian Liang, Xian Guo, Pengjia Bao, Min Chu, Xuezhi Ding, and Ping Yan. 2021. "Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation" Biology 10, no. 10: 949. https://doi.org/10.3390/biology10100949