Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) Release Reactive Oxygen Species (ROS) as Secondary Mn(II) Oxidation Mechanisms to Avoid Toxicity
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Soil Sampling
2.2. Bacterial Isolation and Identification
2.3. Bacterial Identification by MALDITOF-TOF
2.4. Inoculum Preparation and Temperature Experiment
2.5. Quantification of Mn(III/IV) and Bacterial Growth Kinetics
2.6. Peroxidase Activity and ROS Production
2.7. Contribution of ROS Production on Mn(II) Oxidation
2.8. Influence of Mn(II) on Membrane Potential, Viability, and Bacterial Morphology
2.9. Statistical Analysis
3. Results
3.1. Manganese Oxidizing Bacteria in Antarctic Soils
3.2. Effect of Temperature on Bacterial Growth and Mn(II) Oxidation
3.3. Peroxidase Activity and ROS Production under Increased Temperature
3.4. Influence of ROS Production on Mn Oxidation
3.5. Scanning Electron Microscopy
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SITE | APB 1 | MnOxb 2 | Strain Code | Closest Related Species | Class | log (Score) 3 |
---|---|---|---|---|---|---|
S1 | 5 × 105 ± 0.48 | 2 × 102 ± 0.73 | B1 | Microbacterium esteraromaticum | Actinobacteria | 2.34 |
S2 | 2.1 × 105 ± 0.35 | 2.3 × 102 ± 0.89 | B2 | Pseudomonas extremorientalis | Gammaproteobacteria | 2.17 |
S2 | 2.1 × 105 ± 0.35 | 2.3 × 102 ± 0.89 | B3 | Variovorax paradoxus | Betaproteobacteria | 2.15 |
S3 | 4.4 × 105 ± 1.18 | 2.2 × 102 ± 0.27 | B4 | Arthrobacter psychrolactophilus | Actinobacteria | 2.09 |
S3 | 4.4 × 105 ± 1.18 | 2.2 × 102 ± 0.27 | B5 | Chryseobacterium indoltheticum | Flavobacteria | 2.36 |
S4 | 3.7 × 105 ± 0.20 | 2.7 × 102 ± 0.52 | B6 | Chryseobacterium chaponense | Flavobacteria | 2.13 |
S5 | 6.7 × 106 ± 0.46 | 6.2 × 103 ± 0.72 | B7 | Arthrobacter oxydans | Actinobacteria | 2.16 |
S6 | 1.5 × 106 ± 0.31 | 9.4 × 103 ± 0.33 | B8 | Rhodococcus erythropolis | Actinobacteria | 2.14 |
S7 | 1.7 × 106 ± 0.26 | 2.3 × 102 ± 0.64 | B9 | Arthrobacter arylaitensis | Actinobacteria | 2.23 |
S8 | 6.8 × 106 ± 0.46 | 6.5 × 103 ± 0.24 | B10 | Bacillus megaterium | Bacilli | 2.11 |
S9 | 8.7 × 106 ± 0.55 | 2.4 × 103 ± 0.62 | B11 | Lactobacillus plantarum | Bacilli | 2.17 |
S10 | 6.7 × 106 ± 0.46 | 6.2 × 103 ± 0.72 | B12 | Bacillus weihenstephanensis | Bacilli | 2.18 |
S10 | 9.2 × 106 ± 0.31 | 7.8 × 102 ± 0.38 | B13 | Rhodococcus fascians | Actinobacteria | 2.16 |
S10 | 6.7 × 106 ± 0.46 | 6.2 × 103 ± 0.72 | B14 | Sphingomonas echinoides | Alphaproteobacteria | 2.19 |
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Jofré, I.; Matus, F.; Mendoza, D.; Nájera, F.; Merino, C. Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) Release Reactive Oxygen Species (ROS) as Secondary Mn(II) Oxidation Mechanisms to Avoid Toxicity. Biology 2021, 10, 1004. https://doi.org/10.3390/biology10101004
Jofré I, Matus F, Mendoza D, Nájera F, Merino C. Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) Release Reactive Oxygen Species (ROS) as Secondary Mn(II) Oxidation Mechanisms to Avoid Toxicity. Biology. 2021; 10(10):1004. https://doi.org/10.3390/biology10101004
Chicago/Turabian StyleJofré, Ignacio, Francisco Matus, Daniela Mendoza, Francisco Nájera, and Carolina Merino. 2021. "Manganese-Oxidizing Antarctic Bacteria (Mn-Oxb) Release Reactive Oxygen Species (ROS) as Secondary Mn(II) Oxidation Mechanisms to Avoid Toxicity" Biology 10, no. 10: 1004. https://doi.org/10.3390/biology10101004