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Enhanced Macrophage Tribbles-1 Expression in Murine Experimental Atherosclerosis

Department of Cardiovascular Science, University of Sheffield, Sheffield, S10 2RX, UK
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biology 2012, 1(1), 43-57;
Received: 1 March 2012 / Revised: 13 March 2012 / Accepted: 31 March 2012 / Published: 10 April 2012
Development of the atherosclerotic plaque involves a complex interplay between a number of cell types and an extensive inter-cellular communication via cell bound as well as soluble mediators. The family of tribbles proteins has recently been identified as novel controllers of pro-inflammatory signal transduction. The objective of this study was to address the expression pattern of all three tribbles proteins in atherosclerotic plaques from a mouse model of atherosclerosis. Each tribbles were expressed in vascular smooth muscle cells, endothelial cells as well as in resident macrophages of mouse atherosclerotic plaques. The role of IL-1 mediated inflammatory events in controlling tribbles expression was also addressed by inducing experimental atherosclerosis in ApoE−/−IL1R1−/− (double knockout) mice. Immunohistochemical analysis of these mice showed a selective decrease in the percentage of trb-1 expressing macrophages, compared to the ApoE−/− cohort (14.7% ± 1.55 vs. 26.3% ± 1.19). The biological significance of this finding was verified in vitro where overexpression of trb-1 in macrophages led to a significant attenuation (~70%) of IL-6 production as well as a suppressed IL-12 expression induced by a proinflammatory stimulus. In this in vitro setting, expression of truncated trb-1 mutants suggests that the kinase domain of this protein is sufficient to exert this inhibitory action. View Full-Text
Keywords: Tribbles; atherosclerosis; macrophages; mouse Tribbles; atherosclerosis; macrophages; mouse
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MDPI and ACS Style

Sung, H.Y.; Francis, S.E.; Arnold, N.D.; Holland, K.; Ernst, V.; Angyal, A.; Kiss-Toth, E. Enhanced Macrophage Tribbles-1 Expression in Murine Experimental Atherosclerosis. Biology 2012, 1, 43-57.

AMA Style

Sung HY, Francis SE, Arnold ND, Holland K, Ernst V, Angyal A, Kiss-Toth E. Enhanced Macrophage Tribbles-1 Expression in Murine Experimental Atherosclerosis. Biology. 2012; 1(1):43-57.

Chicago/Turabian Style

Sung, Hye Youn, Sheila E. Francis, Nadine D. Arnold, Karen Holland, Vanessa Ernst, Adrienn Angyal, and Endre Kiss-Toth. 2012. "Enhanced Macrophage Tribbles-1 Expression in Murine Experimental Atherosclerosis" Biology 1, no. 1: 43-57.

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