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Fibers 2018, 6(2), 33; https://doi.org/10.3390/fib6020033

Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages

1
Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain
2
Center for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, Georgia
3
Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, # 240 David Aghmashenebeli Alley, Tblisi 0159, Georgia
4
Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany, 10-14, 08019 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
Received: 2 April 2018 / Revised: 7 May 2018 / Accepted: 9 May 2018 / Published: 23 May 2018
(This article belongs to the Special Issue Antimicrobial Electrospun Fibers)
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Abstract

The capacity to load bacteriophages into electrospun nanofibers of two representative biocompatible polymers has been evaluated, paying special attention to the possibility of preserving their antibacterial activity. Specifically, the work involves the following steps: (a) Evaluation of the effect of the applied electrical field on the phage activity; (b) evaluation of the activity when a lyophilization process could be avoided by using water soluble polymers (e.g., poly(ethylene glycol); (c) evaluation of the activity when dissolution of the polymer requires an organic solvent and lyophilization is theoretically necessary. In this case, a poly(ester urea) (PEU) derived from the natural L-leucine amino acid has been considered. Adsorption of commercial bacteriophage preparations into calcium carbonate particles was demonstrated to be a promising methodology to avoid lyophilization and keep the initial bactericide activity at a maximum. Phagestaph and Fersis bacteriophage commercial preparations have been selected for this study due to their specific activity against Staphylococci (e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria. Adhesion and proliferation assays using epithelial cells demonstrated the biocompatibility of both unloaded and bacteriophage-loaded PEU scaffolds, although some slight differences were observed depending on the type of bacteriophage and the selected preparation methodology. View Full-Text
Keywords: bacteriophages; electrospinning; scaffolds; poly(ester urea)s; bactericides; biocompatibility bacteriophages; electrospinning; scaffolds; poly(ester urea)s; bactericides; biocompatibility
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Díaz, A.; del Valle, L.J.; Rodrigo, N.; Casas, M.T.; Chumburidze, G.; Katsarava, R.; Puiggalí, J. Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages. Fibers 2018, 6, 33.

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