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Open AccessArticle

Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern

1
Departamento de Sanidad Animal, Unidad de Microbiología e Inmunología, Universidad de León, s/n, 24071 León, Spain
2
Grup de Sanejament Porcí, 25192 Lleida, Spain
3
Departament de Ciència Animal, ETSEA, Universitat de Lleida-Agrotecnio, 25198 Lleida, Spain
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(9), 614; https://doi.org/10.3390/antibiotics9090614
Received: 2 September 2020 / Revised: 15 September 2020 / Accepted: 16 September 2020 / Published: 17 September 2020
Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected from farms in “Castilla y León” (north-western Spain) in 2017–2019. These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown against tetracyclines (52.1% for doxycycline, 68.7% for oxytetracycline), sulphamethoxazole/trimethoprim (43.7%) and tiamulin (25.0%), thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. Overall, 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none were positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. The ermC gene was observed in 41.7% of isolates, a percentage that decreased to 22.9% for msrE; finally, ermA was harbored by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed. View Full-Text
Keywords: Pasteurella multocida; antimicrobial resistance genes; antimicrobial susceptibility patterns; swine Pasteurella multocida; antimicrobial resistance genes; antimicrobial susceptibility patterns; swine
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Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics 2020, 9, 614.

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