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Open AccessArticle

Validation of a Worst-Case Scenario Method Adapted to the Healthcare Environment for Testing the Antibacterial Effect of Brass Surfaces and Implementation on Hospital Antibiotic-Resistant Strains

1
Laboratoire AGIR - UR UPJV 4294, UFR de Pharmacie, Université de Picardie Jules Verne, 80037 Amiens, France
2
FAVI Limited Company, 80490 Hallencourt, France
3
Laboratoire Hygiène, Risque Biologique et Environnement, CHU Amiens-Picardie, 80025 Amiens, France
*
Author to whom correspondence should be addressed.
Antibiotics 2020, 9(5), 245; https://doi.org/10.3390/antibiotics9050245
Received: 18 April 2020 / Revised: 6 May 2020 / Accepted: 9 May 2020 / Published: 12 May 2020
(This article belongs to the Special Issue Antimicrobial Materials and Surface)
The evaluation of antibacterial activity of metal surfaces can be carried out using various published guidelines which do not always agree with each other on technical conditions and result interpretation. Moreover, these technical conditions are sometimes remote from real-life ones, especially those found in health-care facilities, and do not include a variety of antibiotic-resistant strains. A worst-case scenario protocol adapted from published guidelines was validated on two reference strains (Staphylococcus aureus ATCC 6538 and Enterobacter aerogenes ATCC 13048). This protocol was designed to be as close as possible to a healthcare facility environment, including a much shorter exposure-time than the one recommended in guidelines, and evaluated the impact of parameters such as the method used to prepare inocula, seed on the surface, and recover bacteria following exposure. It was applied to a panel of 12 antibiotic-resistant strains (methicillin resistant, vancomycin-resistant, beta-lactamase, and carbapenemase producing strains as well as efflux pump-overexpressing ones) chosen as representative of the main bacteria causing hospital acquired infections. Within a 5-min exposure time, the tested brass surface displayed an antibacterial effect meeting a reduction cut-off of 99% compared to stainless steel, whatever the resistance mechanism harbored by the bacteria. View Full-Text
Keywords: copper; brass; antibacterial efficiency; hospital acquired infections; antibiotic resistance; antibacterial surfaces copper; brass; antibacterial efficiency; hospital acquired infections; antibiotic resistance; antibacterial surfaces
MDPI and ACS Style

Dauvergne, E.; Lacquemant, C.; Adjidé, C.; Mullié, C. Validation of a Worst-Case Scenario Method Adapted to the Healthcare Environment for Testing the Antibacterial Effect of Brass Surfaces and Implementation on Hospital Antibiotic-Resistant Strains. Antibiotics 2020, 9, 245. https://doi.org/10.3390/antibiotics9050245

AMA Style

Dauvergne E, Lacquemant C, Adjidé C, Mullié C. Validation of a Worst-Case Scenario Method Adapted to the Healthcare Environment for Testing the Antibacterial Effect of Brass Surfaces and Implementation on Hospital Antibiotic-Resistant Strains. Antibiotics. 2020; 9(5):245. https://doi.org/10.3390/antibiotics9050245

Chicago/Turabian Style

Dauvergne, Emilie; Lacquemant, Corinne; Adjidé, Crespin; Mullié, Catherine. 2020. "Validation of a Worst-Case Scenario Method Adapted to the Healthcare Environment for Testing the Antibacterial Effect of Brass Surfaces and Implementation on Hospital Antibiotic-Resistant Strains" Antibiotics 9, no. 5: 245. https://doi.org/10.3390/antibiotics9050245

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