Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes
Abstract
:1. Introduction
2. Results
2.1. Potent Inhibition of Reverse Transcriptase by Certain Aminoglycoside Variants
2.2. AG variants Bind Nucleic Acids in High-Molecular-Weight Complexes
3. Discussion
4. Materials and Methods
4.1. Biochemical Reagents
4.2. Primer Extension Assays
4.3. Binding Experiments
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Compounds | IC50 (µM) | ||
---|---|---|---|
WT | K103N | Y181C | |
NVP | 0.3 ± 0.2 | 180 ± 40 | 200 ± 30 |
TOB-C12 | 0.3 ± 0.1 | 0.6 ± 0.1 | 0.5 ± 0.1 |
TOB-C14 | 1.9 ± 0.3 | 1.2 ± 0.6 | 1.4 ± 0.4 |
TOB-Nap | 40 ± 5 | 40 ± 11 | 12 ± 4 |
KANB-C8 | 8 ± 3 | 7 ± 1 | 8 ± 2 |
KANB-C10 | 3.8 ± 0.3 | 1.9 ± 0.3 | 1.0 ± 0.6 |
KANB-C12 | 1.9 ± 0.3 | 2.0 ± 0.2 | 0.5 ± 0.3 |
KANB-C14 | 1.0 ± 0.1 | 0.9 ± 0.1 | 0.7 ± 0.3 |
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Ying, L.; Zhu, H.; Fosso, M.Y.; Garneau-Tsodikova, S.; Fredrick, K. Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes. Antibiotics 2020, 9, 93. https://doi.org/10.3390/antibiotics9020093
Ying L, Zhu H, Fosso MY, Garneau-Tsodikova S, Fredrick K. Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes. Antibiotics. 2020; 9(2):93. https://doi.org/10.3390/antibiotics9020093
Chicago/Turabian StyleYing, Lanqing, Hongkun Zhu, Marina Y. Fosso, Sylvie Garneau-Tsodikova, and Kurt Fredrick. 2020. "Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes" Antibiotics 9, no. 2: 93. https://doi.org/10.3390/antibiotics9020093