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Open AccessArticle

Influence of Storage on the Antimicrobial and Cytotoxic Activities of a Nisin-biogel with Potential to be Applied to Diabetic Foot Infections Treatment

CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1049-001 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Antibiotics 2020, 9(11), 781; https://doi.org/10.3390/antibiotics9110781
Received: 9 October 2020 / Revised: 29 October 2020 / Accepted: 3 November 2020 / Published: 6 November 2020
(This article belongs to the Special Issue Peptide Antibiotics from Microbes and Venomous Animals)
Staphylococcus aureus is the most prevalent pathogen in diabetic foot infections (DFIs). In addition to its ability to express several virulence factors, including the formation of recalcitrant biofilms, S. aureus is also becoming increasingly resistant to most antibiotics used in clinical practice. The search for alternative treatment strategies for DFI is urgently needed. Antimicrobial peptides (AMPs), namely, nisin, are emerging as potential new therapeutics for managing DFIs. Our team has developed a nisin-guar gum biogel to be applied to DFIs. In this study, to confirm its future in vivo applicability, we evaluated the influence of four storage temperatures (−20 °C, 4 °C, 22 °C, and 37 °C) during a 24 months storage period on its antimicrobial activity towards DFI S. aureus, and its cytotoxicity, to a human keratinocyte cell line. When stored at temperatures below 22 °C, the biogel antimicrobial activity was not significantly influenced by storage duration or temperature. Moreover, nisin incorporated within the guar gum biogel exhibited no significant levels of cytotoxicity on human keratinocyte cells, confirming its potential for DFIs therapeutics. In conclusion, results confirm that the nisin-biogel is a potential candidate to be used as an alternative or complement compound for conventional DFI therapeutics. View Full-Text
Keywords: antimicrobial peptide; nisin; Staphylococcus aureus; diabetic foot ulcers; diabetic foot infections; guar gum; cytotoxicity antimicrobial peptide; nisin; Staphylococcus aureus; diabetic foot ulcers; diabetic foot infections; guar gum; cytotoxicity
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MDPI and ACS Style

Soares, R.S.; Santos, R.; Cunha, E.; Tavares, L.; Trindade, A.; Oliveira, M. Influence of Storage on the Antimicrobial and Cytotoxic Activities of a Nisin-biogel with Potential to be Applied to Diabetic Foot Infections Treatment. Antibiotics 2020, 9, 781. https://doi.org/10.3390/antibiotics9110781

AMA Style

Soares RS, Santos R, Cunha E, Tavares L, Trindade A, Oliveira M. Influence of Storage on the Antimicrobial and Cytotoxic Activities of a Nisin-biogel with Potential to be Applied to Diabetic Foot Infections Treatment. Antibiotics. 2020; 9(11):781. https://doi.org/10.3390/antibiotics9110781

Chicago/Turabian Style

Soares, Rui S.; Santos, Raquel; Cunha, Eva; Tavares, Luís; Trindade, Alexandre; Oliveira, Manuela. 2020. "Influence of Storage on the Antimicrobial and Cytotoxic Activities of a Nisin-biogel with Potential to be Applied to Diabetic Foot Infections Treatment" Antibiotics 9, no. 11: 781. https://doi.org/10.3390/antibiotics9110781

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