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Directed Evolution of a Mycobacteriophage

Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 Paterna, Valencia, Spain
Department of Genetics, Universitat de València, 46100 Burjassot, Valencia, Spain
Author to whom correspondence should be addressed.
Antibiotics 2019, 8(2), 46;
Received: 10 April 2019 / Revised: 22 April 2019 / Accepted: 23 April 2019 / Published: 25 April 2019
(This article belongs to the Special Issue Bacteriophages: Alternatives to Antibiotics and Beyond)
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Bacteriophages represent an alternative strategy to combat pathogenic bacteria. Currently, Mycobacterium tuberculosis infections constitute a major public health problem due to extensive antibiotic resistance in some strains. Using a non-pathogenic species of the same genus as an experimental model, Mycobacterium smegmatis, here we have set up a basic methodology for mycobacteriophage growth and we have explored directed evolution as a tool for increasing phage infectivity and lytic activity. We demonstrate mycobacteriophage adaptation to its host under different conditions. Directed evolution could be used for the development of future phage therapy applications against mycobacteria. View Full-Text
Keywords: phage therapy; Mycobacterium smegmatis; mycobacteriophages; directed evolution phage therapy; Mycobacterium smegmatis; mycobacteriophages; directed evolution

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Cebriá-Mendoza, M.; Sanjuán, R.; Domingo-Calap, P. Directed Evolution of a Mycobacteriophage. Antibiotics 2019, 8, 46.

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