Next Article in Journal
Colonization Resistance of the Gut Microbiota against Clostridium difficile
Previous Article in Journal
Participation of S. Typhimurium cysJIH Operon in the H2S-mediated Ciprofloxacin Resistance in Presence of Sulfate as Sulfur Source
Open AccessArticle

A Clinical Drug Library Screen Identifies Tosufloxacin as Being Highly Active against Staphylococcus aureus Persisters

1
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
2
Lanzhou Center for Tuberculosis Research and Institute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
3
Key Laboratory of Medical Molecular Virology, Department of Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Christopher C. Butler
Antibiotics 2015, 4(3), 329-336; https://doi.org/10.3390/antibiotics4030329
Received: 19 May 2015 / Revised: 5 July 2015 / Accepted: 27 July 2015 / Published: 31 July 2015
To identify effective compounds that are active against Staphylococcus aureus (S. aureus) persisters, we screened a clinical drug library consisting of 1524 compounds and identified six drug candidates that had anti-persister activity: tosufloxacin, clinafloxacin, sarafloxacin, doxycycline, thiostrepton, and chlorosalicylanilide. Among them, tosufloxacin had the highest anti-persister activity, which could completely eradicate S. aureus persisters within 2 days in vitro. Clinafloxacin ranked the second with very few persisters surviving the drug exposure. Interestingly, we found that both tosufloxacin and trovafloxacin that had high activity against persisters contained at the N-1 position the 2,4-difluorophenyl group, which is absent in other less active quinolones and may be associated with the high anti-persister activity. Further studies are needed to evaluate tosufloxacin in animal models and to explain its unique activity against bacterial persisters. Our findings may have implications for improved treatment of persistent bacterial infections. View Full-Text
Keywords: Staphylococcus aureus; persisters; clinical drug library; tosufloxacin; clinafloxacin Staphylococcus aureus; persisters; clinical drug library; tosufloxacin; clinafloxacin
Show Figures

Figure 1

MDPI and ACS Style

Niu, H.; Cui, P.; Yee, R.; Shi, W.; Zhang, S.; Feng, J.; Sullivan, D.; Zhang, W.; Zhu, B.; Zhang, Y. A Clinical Drug Library Screen Identifies Tosufloxacin as Being Highly Active against Staphylococcus aureus Persisters. Antibiotics 2015, 4, 329-336.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop