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Article

Antimicrobial Susceptibility Profiles and Molecular Characterisation of Staphylococcus aureus from Pigs and Workers at Farms and Abattoirs in Zambia

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Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka 10101, Zambia
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Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia
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Central Veterinary Research Institute, Lusaka 10101, Zambia
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Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia
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Macha Research Trust, Choma P.O. Box 630166, Zambia
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Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia
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Department of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia
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Department of Pathology and Microbiology, University Teaching Hospitals, Lusaka 10101, Zambia
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Department of Veterinary Epidemiology and Public Health, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, Lilongwe 207203, Malawi
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College of Veterinary Medicine, Haramaya University, Dire Dawa P.O. Box 138, Ethiopia
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Center for Infectious Disease Research Zambia, Lusaka 10101, Zambia
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Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620, USA
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School of Life & Environmental Sciences, University of Lincoln, Lincolnshire LN6 7TS, UK
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Division of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Carlos M. Franco, Pierluigi Di Ciccio, Selene Rubiola and Felice Panebianco
Antibiotics 2022, 11(7), 844; https://doi.org/10.3390/antibiotics11070844
Received: 4 May 2022 / Revised: 30 May 2022 / Accepted: 30 May 2022 / Published: 24 June 2022
Pigs have been shown to be a reservoir for recently emerging livestock-associated Staphylococcus aureus (LA-SA), including methicillin resistant strains in many countries worldwide. However, there is sparse information about LA-SA strains circulating in Zambia. This study investigated the prevalence, phenotypic and genotypic characteristics of S. aureus from pigs and workers at farms and abattoirs handling pigs in Lusaka Province of Zambia. A total of 492 nasal pig swabs, 53 hand and 53 nasal human swabs were collected from farms and abattoirs in selected districts. Standard microbiological methods were used to isolate and determine antimicrobial susceptibility patterns of S. aureus. Polymerase Chain Reaction was used to confirm the species identity and detect antimicrobial resistance and virulence genes of isolates, whereas genetic diversity was evaluated using spa typing. Overall prevalence of S. aureus was 33.1%, 37.8% for pigs and 11.8% for humans. The isolates were resistant to several antibiotics with resistance ranging from 18% to 98% but were all susceptible to vancomycin. Typical LA-SA spa types were detected. The presence of plasmid mediated resistance genes such as tetM (12.8%), other resistance determinants and immune evasion cluster genes among the isolates is of great public health concern. Thus, continuous surveillance of S. aureus using a “One health” approach is warranted to monitor S.aureus infections and spread of antimicrobial resistance. View Full-Text
Keywords: antimicrobial resistance; Staphylococcus aureus; spa typing; swine; Zambia antimicrobial resistance; Staphylococcus aureus; spa typing; swine; Zambia
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MDPI and ACS Style

Samutela, M.T.; Phiri, B.S.J.; Simulundu, E.; Kwenda, G.; Moonga, L.; Bwalya, E.C.; Muleya, W.; Nyirahabimana, T.; Yamba, K.; Kainga, H.; Kallu, S.A.; Mwape, I.; Frey, A.; Bates, M.; Higashi, H.; Hang'ombe, B.M. Antimicrobial Susceptibility Profiles and Molecular Characterisation of Staphylococcus aureus from Pigs and Workers at Farms and Abattoirs in Zambia. Antibiotics 2022, 11, 844. https://doi.org/10.3390/antibiotics11070844

AMA Style

Samutela MT, Phiri BSJ, Simulundu E, Kwenda G, Moonga L, Bwalya EC, Muleya W, Nyirahabimana T, Yamba K, Kainga H, Kallu SA, Mwape I, Frey A, Bates M, Higashi H, Hang'ombe BM. Antimicrobial Susceptibility Profiles and Molecular Characterisation of Staphylococcus aureus from Pigs and Workers at Farms and Abattoirs in Zambia. Antibiotics. 2022; 11(7):844. https://doi.org/10.3390/antibiotics11070844

Chicago/Turabian Style

Samutela, Mulemba Tillika, Bruno Stephen July Phiri, Edgar Simulundu, Geoffrey Kwenda, Ladslav Moonga, Eugene C. Bwalya, Walter Muleya, Therese Nyirahabimana, Kaunda Yamba, Henson Kainga, Simegnew Adugna Kallu, Innocent Mwape, Andrew Frey, Matthew Bates, Hideaki Higashi, and Bernard Mudenda Hang'ombe. 2022. "Antimicrobial Susceptibility Profiles and Molecular Characterisation of Staphylococcus aureus from Pigs and Workers at Farms and Abattoirs in Zambia" Antibiotics 11, no. 7: 844. https://doi.org/10.3390/antibiotics11070844

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