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Article

Derivatives of Esculentin-1 Peptides as Promising Candidates for Fighting Infections from Escherichia coli O157:H7

1
Biological Service, Italian National Institute of Health, 00161 Rome, Italy
2
Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Department of Biochemical Sciences, Sapienza University of Rome, 00185 Rome, Italy
3
National Center for Drug Research and Evaluation, Italian National Institute of Health, 00161 Rome, Italy
*
Authors to whom correspondence should be addressed.
Academic Editors: Jean-Marc Sabatier, Daniele Castagnolo, Jürgen Brem, Mark G. Moloney and Sónia Silva
Antibiotics 2022, 11(5), 656; https://doi.org/10.3390/antibiotics11050656
Received: 17 March 2022 / Revised: 5 May 2022 / Accepted: 11 May 2022 / Published: 13 May 2022
New strategies are needed to fight the emergence of multidrug-resistant bacteria caused by an overuse of antibiotics in medical and veterinary fields. Due to the importance of biofilms in clinical infections, antibiofilm peptides have a great potential to treat infections. In recent years, an increased interest has emerged in antimicrobial peptides (AMPs). One of the richest sources of AMPs is represented by amphibian skin. In the present work, we investigated the effects of two peptides derived from the frog skin AMP esculentin-1, namely, Esc(1-21) and Esc(1-18), on the growth, biofilm formation, and gene expression of the non-pathogenic Escherichia coli strain K12 and of enterohemorrhagic E. coli O157:H7. Both peptides showed minimal bactericidal concentrations ranging from 4 to 8 µM for Esc(1-21) and from 32 to 64 µM for Esc(1-18). They also, at sub-MIC doses, reduced the formation of biofilm, as supported by both microbiological assays and scanning electron microscopy, while they displayed no marked activity against the planktonic form of the bacteria. Transcriptional analysis in E. coli O157:H7 showed that both AMPs induced the expression of several genes involved in the regulation of formation and dispersal of biofilm, as well as in the stress response. In conclusion, we demonstrated that these AMPs affect E. coli O157:H7 growth and biofilm formation, thus suggesting a great potential to be developed as novel therapeutics against infections caused by bacterial biofilms. View Full-Text
Keywords: antimicrobial peptide; esculentin; biofilm; Escherichia coli O157:H7; antibacterial activity; antibiofilm antimicrobial peptide; esculentin; biofilm; Escherichia coli O157:H7; antibacterial activity; antibiofilm
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MDPI and ACS Style

Scotti, R.; Casciaro, B.; Stringaro, A.; Morgia, F.; Mangoni, M.L.; Gabbianelli, R. Derivatives of Esculentin-1 Peptides as Promising Candidates for Fighting Infections from Escherichia coli O157:H7. Antibiotics 2022, 11, 656. https://doi.org/10.3390/antibiotics11050656

AMA Style

Scotti R, Casciaro B, Stringaro A, Morgia F, Mangoni ML, Gabbianelli R. Derivatives of Esculentin-1 Peptides as Promising Candidates for Fighting Infections from Escherichia coli O157:H7. Antibiotics. 2022; 11(5):656. https://doi.org/10.3390/antibiotics11050656

Chicago/Turabian Style

Scotti, Raffaella, Bruno Casciaro, Annarita Stringaro, Fabrizio Morgia, Maria L. Mangoni, and Roberta Gabbianelli. 2022. "Derivatives of Esculentin-1 Peptides as Promising Candidates for Fighting Infections from Escherichia coli O157:H7" Antibiotics 11, no. 5: 656. https://doi.org/10.3390/antibiotics11050656

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