Next Article in Journal
Molecular Weight Identification of Compounds Involved in the Fungal Synthesis of AgNPs: Effect on Antimicrobial and Photocatalytic Activity
Previous Article in Journal
Screening of Natural Molecules as Adjuvants to Topical Antibiotics to Treat Staphylococcus aureus from Diabetic Foot Ulcer Infections
Previous Article in Special Issue
Assessing Knowledge, Beliefs, and Behaviors around Antibiotic Usage and Antibiotic Resistance among UK Veterinary Students: A Multi-Site, Cross-Sectional Survey
Article

Mec-Positive Staphylococcus Healthcare-Associated Infections Presenting High Transmission Risks for Antimicrobial-Resistant Strains in an Equine Hospital

1
Escuela de Medicina Veterinaria, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago 9340000, Chile
2
Department of Disease Control, Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London WC1E 7HT, UK
3
College of Medicine and Health, University of Exeter, Exeter EX1 2LU, UK
4
Institute for Global Health, University College London, London WC1N 1EH, UK
5
Laboratorio de Investigación en Agentes Antibacterianos, Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción P.O. Box C-160, Chile
6
Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepción, Concepción 4030000, Chile
7
Escuela de Medicina, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago 8940000, Chile
8
Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal, Facultad de Ciencias Biológicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago 8940000, Chile
*
Authors to whom correspondence should be addressed.
Academic Editors: Nichola R. Naylor and Chris Pinto J.
Antibiotics 2022, 11(5), 621; https://doi.org/10.3390/antibiotics11050621
Received: 1 April 2022 / Revised: 24 April 2022 / Accepted: 27 April 2022 / Published: 4 May 2022
Healthcare-associated infections caused by Staphylococcus, particularly Staphylococcus aureus, represent a high risk for human and animal health. Staphylococcus can be easily transmitted through direct contact with individual carriers or fomites, such as medical and non-medical equipment. The risk increases if S. aureus strains carry antibiotic resistance genes and show a phenotypic multidrug resistance behavior. The aim of the study was to identify and characterize methicillin resistant coagulase-positive staphylococci (MRSA) and coagulase-negative staphylococci (MRCoNS) in equine patients and environmental sources in an equine hospital to evaluate the genetic presence of multidrug resistance and to understand the dissemination risks within the hospital setting. We explored 978 samples for MRSA and MRCoNS using Oxacillin Screen Agar in an equine hospital for racehorses in Chile, which included monthly samples (n = 61–70) from equine patients (246) and hospital environments (732) in a one-year period. All isolates were PCR-assessed for the presence of methicillin resistance gene mecA and/or mecC. Additionally, we explored the epidemiological relatedness by Pulsed Field Gel Electrophoresis (PFGE) in MRSA isolates. Phenotypic antibiotic resistance was evaluated using the Kirby-Bauer disk diffusion method. We estimated the unadjusted and adjusted risk of acquiring drug-resistant Staphylococcus strains by employing logistic regression analyses. We identified 16 MRSA isolates and 36 MRCoNS isolates. For MRSA, we detected mecA and mecC in 100% and 87.5 % of the isolates, respectively. For MRCoNS, mecA was detected among 94% of the isolates and mecC among 86%. MRSA and MRCoNS were isolated from eight and 13 equine patients, respectively, either from colonized areas or compromised wounds. MRSA strains showed six different pulse types (i.e., A1–A3, B1–B2, C) isolated from different highly transited areas of the hospital, suggesting potential transmission risks for other patients and hospital staff. The risk of acquiring drug-resistant Staphylococcus species is considerably greater for patients from the surgery, equipment, and exterior areas posing higher transmission risks. Tackling antimicrobial resistance (AMR) using a One Health perspective should be advocated, including a wider control over antimicrobial consumption and reducing the exposure to AMR reservoirs in animals, to avoid cross-transmission of AMR Staphylococcus within equine hospitals. View Full-Text
Keywords: methicillin resistance; one health; antimicrobial-resistance; healthcare-associated infections; Staphylococcus methicillin resistance; one health; antimicrobial-resistance; healthcare-associated infections; Staphylococcus
Show Figures

Figure 1

MDPI and ACS Style

Soza-Ossandón, P.; Rivera, D.; Allel, K.; González-Rocha, G.; Quezada-Aguiluz, M.; San Martin, I.; García, P.; Moreno-Switt, A.I. Mec-Positive Staphylococcus Healthcare-Associated Infections Presenting High Transmission Risks for Antimicrobial-Resistant Strains in an Equine Hospital. Antibiotics 2022, 11, 621. https://doi.org/10.3390/antibiotics11050621

AMA Style

Soza-Ossandón P, Rivera D, Allel K, González-Rocha G, Quezada-Aguiluz M, San Martin I, García P, Moreno-Switt AI. Mec-Positive Staphylococcus Healthcare-Associated Infections Presenting High Transmission Risks for Antimicrobial-Resistant Strains in an Equine Hospital. Antibiotics. 2022; 11(5):621. https://doi.org/10.3390/antibiotics11050621

Chicago/Turabian Style

Soza-Ossandón, Paula, Dácil Rivera, Kasim Allel, Gerardo González-Rocha, Mario Quezada-Aguiluz, Ivan San Martin, Patricia García, and Andrea I. Moreno-Switt. 2022. "Mec-Positive Staphylococcus Healthcare-Associated Infections Presenting High Transmission Risks for Antimicrobial-Resistant Strains in an Equine Hospital" Antibiotics 11, no. 5: 621. https://doi.org/10.3390/antibiotics11050621

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop