Comparison of Fitness Cost, Stability, and Conjugation Frequencies of tet(X4)-Positive Plasmids in Chicken and Pig Escherichia coli
Abstract
:1. Introduction
2. Results
2.1. Fitness Cost of the Tet(X4)-Bearing Plasmids in Engineered Bacteria TOP10, Pig E. coli, and Chicken E. coli
2.2. Biofilm Formation of the Tested Strains after Acquiring Various Tet(X4)-Bearing Plasmids
2.3. Swimming Motility of the Tested Strains after Acquiring Various Tet(X4)-Bearing Plasmids
2.4. Effect of Tetracycline on the Stability of Tet(X4)-Bearing Plasmids
2.5. Effect of Temperature on Conjugation Frequencies of the Tet(X4)-Bearing Plasmids
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Plasmids
4.2. Construction of Transformants
4.3. Pairwise Competition Assay
4.4. Biofilm Formation Assay
4.5. Motility Test
4.6. Stability of Tet(X4)-Bearing Plasmids during Serial Passaging
4.7. Conjugation Frequencies of Tet(X4)-Bearing Plasmids at 37 °C and 42 °C
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strains | C3 (Pig) | C41 (Pig) | C42 (Pig) | C54 (Pig) | C81 (Pig) | 11W (Chicken) |
---|---|---|---|---|---|---|
TOP10 | TOP10:C3 | TOP10:C41 | TOP10:C42 | TOP10:C54 | TOP10:C81 | TOP10:11W |
SEC10 (pig) | SEC10:C3 | SEC10:C41 | SEC10:C42 | SEC10:C54 | F | SEC10:11W |
SEC78 (pig) | SEC78:C3 | SEC78:C41 | SEC78:C42 | SEC78:C54 | F | F |
E847 (chicken) | E847:C3 | E847:C41 | E847:C42 | E847:C54 | F | E847:11W |
E901 (chicken) | E901:C3 | E901:C41 | E901:C42 | E901:C54 | E901:C81 | F |
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Liu, Z.; Zhang, H.; Xiao, X.; Liu, Y.; Li, R.; Wang, Z. Comparison of Fitness Cost, Stability, and Conjugation Frequencies of tet(X4)-Positive Plasmids in Chicken and Pig Escherichia coli. Antibiotics 2022, 11, 1657. https://doi.org/10.3390/antibiotics11111657
Liu Z, Zhang H, Xiao X, Liu Y, Li R, Wang Z. Comparison of Fitness Cost, Stability, and Conjugation Frequencies of tet(X4)-Positive Plasmids in Chicken and Pig Escherichia coli. Antibiotics. 2022; 11(11):1657. https://doi.org/10.3390/antibiotics11111657
Chicago/Turabian StyleLiu, Ziyi, Huiru Zhang, Xia Xiao, Yuan Liu, Ruichao Li, and Zhiqiang Wang. 2022. "Comparison of Fitness Cost, Stability, and Conjugation Frequencies of tet(X4)-Positive Plasmids in Chicken and Pig Escherichia coli" Antibiotics 11, no. 11: 1657. https://doi.org/10.3390/antibiotics11111657