Detection of an Enterococcus faecium Carrying a Double Copy of the PoxtA Gene from Freshwater River, Italy
Abstract
:1. Introduction
2. Results
2.1. Detection of Oxazolidinone Resistance Genes in Florfenicol-Resistant Enterococci and Antimicrobial Susceptibility Profiles
2.2. Location of PoxtA Gene and Detection of Circular Forms
2.3. Transferability in Filter Mating Experiments and in Aquaria Microcosm Assays
2.4. WGS Analysis
3. Materials and Methods
3.1. Sampling, Sample Processing, and Enterococcal Isolation
3.2. Genotypic and Phenotypic Characterization
3.3. S1-PFGE, Southern Blotting, and Hybridization Assays
3.4. Detection of Circular Forms
3.5. Mating Experiments
3.6. WGS and Sequence Analysis
3.7. Nucleotide Sequence Accession Numbers
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Recipient | Transfer Frequency | Transconjugants e MIC (mg/L) of: f | |||||||
---|---|---|---|---|---|---|---|---|---|
FFC | CHL | LZD | TZD | TE | ERY | ||||
Filter mating experiments | |||||||||
E. faecium 64/3 | 5.8 × 10−2 | 64 | 32 | 4 | 0.5 | 128 | 128 | ||
E. faecium Ef1 | 5 × 10−3 | 128 | 64 | 8 | 1 | 128 | >128 | ||
Aquaria microcosm assays | |||||||||
Aquarium A a | 24 h | E. faecium 64/3 | 6.3 × 10−5 | 64 | 32 | 4 | 0.5 | 128 | >128 |
48 h | E. faecium 64/3 | 1.8 × 10−7 | 64 | 32 | 4 | 0.5 | 128 | >128 | |
96 h | E. faecium 64/3 | ND d | |||||||
Aquarium B b | 24 h | E. faecium 64/3 | 5 × 10−4 | 64 | 32 | 4 | 0.5 | 128 | >128 |
48 h | E. faecium 64/3 | 2.7 × 10−7 | 64 | 32 | 4 | 0.5 | 128 | >128 | |
96 h | E. faecium 64/3 | ND | |||||||
Aquarium C c | 24 h | E. faecium 64/3 | 9.2 × 10−4 | 64 | 32 | 4 | 0.5 | 128 | >128 |
48 h | E. faecium 64/3 | 2.9 × 10−7 | 64 | 32 | 4 | 0.5 | 128 | >128 | |
96 h | E. faecium 64/3 | ND |
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Cinthi, M.; Coccitto, S.N.; Morroni, G.; D’Achille, G.; Brenciani, A.; Giovanetti, E. Detection of an Enterococcus faecium Carrying a Double Copy of the PoxtA Gene from Freshwater River, Italy. Antibiotics 2022, 11, 1618. https://doi.org/10.3390/antibiotics11111618
Cinthi M, Coccitto SN, Morroni G, D’Achille G, Brenciani A, Giovanetti E. Detection of an Enterococcus faecium Carrying a Double Copy of the PoxtA Gene from Freshwater River, Italy. Antibiotics. 2022; 11(11):1618. https://doi.org/10.3390/antibiotics11111618
Chicago/Turabian StyleCinthi, Marzia, Sonia Nina Coccitto, Gianluca Morroni, Gloria D’Achille, Andrea Brenciani, and Eleonora Giovanetti. 2022. "Detection of an Enterococcus faecium Carrying a Double Copy of the PoxtA Gene from Freshwater River, Italy" Antibiotics 11, no. 11: 1618. https://doi.org/10.3390/antibiotics11111618