Imidazothiazole Derivatives Exhibited Potent Effects against Brain-Eating Amoebae
Abstract
:1. Introduction
2. Results
2.1. Majority of Compounds Exhibited Significant Amoebicidal Properties against Naegleria fowleri
2.2. Compounds Exhibited Limited Cytotoxicity against Human Cell Lines
2.3. Majority of Compounds Resulted in Decreased in Amoeba-Mediated Cytotoxic Activity against Human Cells
3. Discussion
4. Materials and Methods
4.1. The Tested Compounds
4.2. Henrietta Lacks (HeLa) Cervical Cancer Cells
4.3. Naegleria Fowleri Culture
4.4. Amoebicidal Assay
4.5. Cytotoxicity Assay
4.6. Cytopathogenicity Assay
4.7. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Naegleria fowleri | ||||
---|---|---|---|---|
25 µM | 50 µM | 75 µM | MIC50 | |
Amoeba Alone | 100 | |||
1e | 93 ± 9.7 | 52.16 ± 1.7 | 19 ± 1.7 | 50.96 |
1f | 99 ± 7.3 | 44 ± 3.9 | 17 ± 4.4 | 48.45 |
1h | 96 ± 8.5 | 73 ± 2.4 | 26 ± 0.8 | 60.87 |
Compound No. | Structure |
---|---|
1a | |
1b | |
1c | |
1d | |
1e | |
1f | |
1g | |
1h | |
1i | |
1j | |
1k | |
1l | |
1m | |
1n | |
1o | |
1p | |
1q | |
1r | |
1s | |
1t | |
1u | |
1v | |
1w | |
1x | |
1y | |
1z | |
1za | |
1zb | |
1zc | |
1zd |
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Siddiqui, R.; El-Gamal, M.I.; Boghossian, A.; Saeed, B.Q.; Oh, C.-H.; Abdel-Maksoud, M.S.; Alharbi, A.M.; Alfahemi, H.; Khan, N.A. Imidazothiazole Derivatives Exhibited Potent Effects against Brain-Eating Amoebae. Antibiotics 2022, 11, 1515. https://doi.org/10.3390/antibiotics11111515
Siddiqui R, El-Gamal MI, Boghossian A, Saeed BQ, Oh C-H, Abdel-Maksoud MS, Alharbi AM, Alfahemi H, Khan NA. Imidazothiazole Derivatives Exhibited Potent Effects against Brain-Eating Amoebae. Antibiotics. 2022; 11(11):1515. https://doi.org/10.3390/antibiotics11111515
Chicago/Turabian StyleSiddiqui, Ruqaiyyah, Mohammed I. El-Gamal, Anania Boghossian, Balsam Qubais Saeed, Chang-Hyun Oh, Mohammed S. Abdel-Maksoud, Ahmad M. Alharbi, Hasan Alfahemi, and Naveed Ahmed Khan. 2022. "Imidazothiazole Derivatives Exhibited Potent Effects against Brain-Eating Amoebae" Antibiotics 11, no. 11: 1515. https://doi.org/10.3390/antibiotics11111515