Next Article in Journal
The Predictive Value of Cell Blood Count Parameters to Diagnose Pulmonary Embolism in Patients with SARS-CoV-2 Infection: A Case Control Study
Next Article in Special Issue
Antimicrobial Activity of Eucalyptus globulus, Azadirachta indica, Glycyrrhiza glabra, Rheum palmatum Extracts and Rhein against Porphyromonas gingivalis
Previous Article in Journal
Biocompatibility and Comfort during Extended Wear of Mel4 Peptide-Coated Antimicrobial Contact Lenses
Article

Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro

1
Department of Microbiology, PSG College of Arts & Science, Coimbatore 641014, India
2
Department of Food Science and Nutrition, King Saud University, Riyadh 11451, Saudi Arabia
3
Central Laboratory, Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
4
Clinical Laboratory Science Department, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia
5
Department of Biology, College of Science and Humanities, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia
6
Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Academic Editor: Marc Maresca
Antibiotics 2022, 11(1), 59; https://doi.org/10.3390/antibiotics11010059
Received: 1 December 2021 / Revised: 24 December 2021 / Accepted: 30 December 2021 / Published: 3 January 2022
(This article belongs to the Special Issue Antimicrobial Activities of Plant Extracts)
The current study aimed to screen the preliminary phytochemicals in the leaf extract of the medicinal plant Simarouba glauca and to analyze its potential antimicrobial, antioxidant and anticancer properties. The phytochemical profile of the methanol extract was analyzed, and bioactive compounds were identified using chromatography, FTIR and GCMS. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were determined against 14 bacterial and 6 fungal strains. Moreover, the synergistic effect of a plant extract with commercially available antibiotics was also evaluated using the checkerboard method. The ethanolic and methanolic extracts showed exclusive activity against S. aureus and profound activity against E. coli and S. marcescens. Upon comparing breakpoints, methanolic extract demonstrated higher antimicrobial activity with a MIC value of 3.2 mg/mL against the test pathogens. Furthermore, the extracts demonstrated potential antioxidant activity; methanol extract had higher antioxidant potential compared to the ethanol extract. The major proactive bioactive compound with maximum antioxidant capacity was observed to be terpenoids. The methanol extract of S. glauca showed significant cytotoxicity against the MCF-7 breast cancer cell line with an IC50 value of 16.12 µg/mL. The overall results of our work provide significant evidence for the usage of methanolic extract of S. glauca as an efficient ethnomedicinal agent and a potential candidate for relieving many human ailments. View Full-Text
Keywords: Simarouba glauca; terpenoids; antimicrobial; antioxidants; anticancer; MCF-7 cell line Simarouba glauca; terpenoids; antimicrobial; antioxidants; anticancer; MCF-7 cell line
Show Figures

Figure 1

MDPI and ACS Style

Ramasamy, S.P.; Rajendran, A.; Pallikondaperumal, M.; Sundararajan, P.; Husain, F.M.; Khan, A.; Hakeem, M.J.; Alyousef, A.A.; Albalawi, T.; Alam, P.; Ali, H.M.; Alqasim, A. Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro. Antibiotics 2022, 11, 59. https://doi.org/10.3390/antibiotics11010059

AMA Style

Ramasamy SP, Rajendran A, Pallikondaperumal M, Sundararajan P, Husain FM, Khan A, Hakeem MJ, Alyousef AA, Albalawi T, Alam P, Ali HM, Alqasim A. Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro. Antibiotics. 2022; 11(1):59. https://doi.org/10.3390/antibiotics11010059

Chicago/Turabian Style

Ramasamy, Shanmuga P., Anitha Rajendran, Muthukrishnan Pallikondaperumal, Priya Sundararajan, Fohad M. Husain, Altaf Khan, Mohammed J. Hakeem, Abdullah A. Alyousef, Thamer Albalawi, Pravej Alam, Hazim M. Ali, and Abdulaziz Alqasim. 2022. "Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro" Antibiotics 11, no. 1: 59. https://doi.org/10.3390/antibiotics11010059

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop