Antifungal Carvacrol Loaded Chitosan Nanoparticles
Abstract
:1. Introduction
2. Results and Discussion
2.1. NPs Preparation
2.2. Bioassay Guided Nanoparticles Optimization
2.3. Cs–Cv-NPs Characterization
2.4. Evaluation of Antimicrobial Activity
2.5. Inhibition on Pre-Formed and Forming Biofilm by Cv–Cs-NPs in Candida spp.
3. Materials and Methods
3.1. Chemicals
3.2. Nanoparticles Preparation
3.3. Nanoparticles Characterization
3.3.1. Scanning Electron Microscopy (SEM)
3.3.2. Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy (ATR-FTIR)
3.3.3. Dynamic Light Scattering
3.3.4. Evaluation of Encapsulation Efficiency and Loading Capacity
3.4. Release Kinetics
3.5. Organisms and Growth Conditions
3.6. Evaluation of Antimicrobial Activity
3.7. In Vitro Time–Kill Kinetics
3.8. Biofilm Formation
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Material | Cs/Cv (w/w) | MIC µg/mL |
---|---|---|
Cs | - | >2400 |
Cv | - | 1200 |
Cs-NPs | - | 1580 |
Cv–Cs NPs | 1:0.25 | >2400 |
Cv–Cs-NPs | 1:0.50 | >2400 |
Cv–Cs-NPs | 1:0.75 | >2400 |
Cv–Cs-NPs | 1:1 | >2400 |
Cv–Cs-NPs | 1:1.25 | 1200 |
Cv–Cs-NPs | 1:1.50 | 24 |
Cv–Cs-NP | Cs-NP | |
---|---|---|
Hydrodynamic diameter (nm) | 281.6 ± 2 | 384.5 ± 3 |
Polydispersity index (PdI) | 0.235 ± 0.03 | 0.2475 ± 0.02 |
Strains | Cs | Cv | Cs-NPs | Cv–Cs-NPs 1:1.50 |
---|---|---|---|---|
C. albicans AIDS68 | 1560 | 780 | 1560 | 24 |
C. tropicalis 47829 | >3120 | 780 | 3120 | 1560 |
C. krusei 45709 | 1560 | 780 | 3120 | 1560 |
C. glabrata SFY115 | 1560 | 780 | 1560 | 780 |
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Vitali, A.; Stringaro, A.; Colone, M.; Muntiu, A.; Angiolella, L. Antifungal Carvacrol Loaded Chitosan Nanoparticles. Antibiotics 2022, 11, 11. https://doi.org/10.3390/antibiotics11010011
Vitali A, Stringaro A, Colone M, Muntiu A, Angiolella L. Antifungal Carvacrol Loaded Chitosan Nanoparticles. Antibiotics. 2022; 11(1):11. https://doi.org/10.3390/antibiotics11010011
Chicago/Turabian StyleVitali, Alberto, Annarita Stringaro, Marisa Colone, Alexandra Muntiu, and Letizia Angiolella. 2022. "Antifungal Carvacrol Loaded Chitosan Nanoparticles" Antibiotics 11, no. 1: 11. https://doi.org/10.3390/antibiotics11010011