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Article

Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes

Photobios–Photobiology Applied to Health, University of Vale do Paraíba, Urbanova, São José dos Campos 2911, SP, Brazil
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Author to whom correspondence should be addressed.
Academic Editor: Kristjan Plaetzer
Antibiotics 2021, 10(6), 634; https://doi.org/10.3390/antibiotics10060634
Received: 31 March 2021 / Revised: 29 April 2021 / Accepted: 29 April 2021 / Published: 25 May 2021
(This article belongs to the Special Issue New and Innovative Applications of Antimicrobial Photodynamic Therapy)
Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 µg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 µg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed. View Full-Text
Keywords: photodynamic therapy; curcumin; amastigotes; macrophages; cutaneous leishmaniasis photodynamic therapy; curcumin; amastigotes; macrophages; cutaneous leishmaniasis
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MDPI and ACS Style

Pereira, A.H.C.; Marcolino, L.M.C.; Pinto, J.G.; Ferreira-Strixino, J. Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes. Antibiotics 2021, 10, 634. https://doi.org/10.3390/antibiotics10060634

AMA Style

Pereira AHC, Marcolino LMC, Pinto JG, Ferreira-Strixino J. Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes. Antibiotics. 2021; 10(6):634. https://doi.org/10.3390/antibiotics10060634

Chicago/Turabian Style

Pereira, André H.C., Luciana M.C. Marcolino, Juliana G. Pinto, and Juliana Ferreira-Strixino. 2021. "Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes" Antibiotics 10, no. 6: 634. https://doi.org/10.3390/antibiotics10060634

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