Abstract
Patients with chronic urinary and urogenital multidrug resistant bacterial infections received phage therapy (PT) using intravesical or intravesical and intravaginal phage administration. A single course of PT did not induce significant serum antibody responses against administered phage. Whilst the second cycle of PT caused a significant increase in antibody levels, they nevertheless remained quite low. These data combined with good therapy results achieved in some patients suggest that this mode of PT may be an efficient means of therapy for urogenital infections and a reliable model for a clinical trial of PT.
1. Introduction
Even though no clinical trial carried out so far has confirmed the true value of phage therapy (PT) in combating multidrug resistant bacterial infections, interest in PT has grown and many new reports claiming its efficacy have been published []. Among those articles are reports from our group describing promising results achieved in patients with urinary tract infections (UTI), especially in patients with chronic bacterial prostatitis [,]. Furthermore, we reported successful use of PT in association with antibiotics in a renal allograft recipient with UTI []. A recently published review on PT in UTI summarized currently available data suggesting that this mode of treatment may be an attractive option for those patients []. Nevertheless, a randomized, placebo-controlled, double-blind clinical trial of intravesical PT for treating UTI in patients undergoing transurethral resection of the prostate was not found to be superior to placebo bladder irrigation []. Therefore, new studies and more data are needed to clarify the value of PT in treating UTI.
2. Materials and Methods
2.1. Patients
Patients (11 women and 4 men) with bacterial chronic urinary or urogenital infections underwent phage therapy (PT) between 2016 and 2019 at HIIET Phage Therapy Unit (PTU). Patients used phage preparations selected for treatment on the basis of phage typing (phages indicating a high lytic activity against the patient’s bacterial strain) intravaginally and intravesically or intravesically according to the protocol “Experimental phage therapy of drug-resistant bacterial infections, including MRSA infections” []. Phages were administered as follows: intravesically 10 mL two times daily using a urinary catheter Nelaton CH-12; intravaginally and intravesically 10 mL by each route of phage administration two times daily. Eleven patients (7 women and 4 men) underwent one cycle of PT and 4 patients (women) underwent the first cycle of PT with intravaginal and intravesical or intravesical administration of phages (Table 1). Four patients (women) were qualified for the second cycle of PT with intravaginal and intravesical or intravesical administration of phage (Table 2). Patients used phages for 3 days in one cycle of PT or in the first cycle of PT. Then there was a break of 20–61 days before the second cycle of PT, where phages were applied for 3 days. Blood was collected before PT, on the third day of PT and after PT. The blood was centrifuged at 1500× g for 10 min and sera were stored at −70 °C. Sera of voluntary blood donors were obtained from the Blood Transfusion Center in Wrocław, Poland. The antiphage activity of sera (AAS) was studied immediately after obtaining sera. Research of AAS in patients undergoing PT at the HIIET PTU and in healthy individuals were performed after obtaining the consent of the Bioethics Committee of the Wrocław Medical University (Poland). All subjects gave written informed consent.
Table 1.
Patients with chronic urinary or urogenital infections undergoing one cycle or the first cycle of phage therapy.
Table 2.
Patients with chronic urinary or urogenital infections undergoing two cycles of phage therapy.
2.2. Bacteriophage Preparations
Patients with chronic urinary or urogenital infections used monovalent lysates of bacteriophage preparations of Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis or Pseudomonas aeruginosa based on phage typing (Table 1 and Table 2). Phage lysates had concentrations of 106–108 plaque forming unit/mL (pfu/mL). Healthy donors did not use bacteriophage preparations, but their sera were examined for the presence of antiphage antibodies against bacteriophage preparations E. coli phage coli 48/D1, K. pneumoniae phage Kl 16/30, E. faecalis phage Entc 15/P and P. aeruginosa phage Ps F8/PsF8.
2.3. Plate Phage Neutralization Test
The level of AAS of patients undergoing phage therapy and control of healthy individuals was tested with the plate phage neutralization test. The AAS research was performed according to the method described earlier []. Undiluted and diluted sera from 1:10 to 1:1500 were tested. A total of 50 µL of the phage with a titer of 1 × 106 pfu/mL were added to each serum dilution (450 µL). Phage titer control was performed by adding 50 µL of the phage with a titer of 1 × 106 pfu/mL to 450 µL of broth. The mixture was incubated for 30 min at 37 °C. After this time, 50 µL of the mixture were taken and added to 4.95 mL of cold broth. Phage titer was examined at the beginning of the phage reaction with the serum and, after the 30 min reaction, by the standard double-agar layer method. A total of 100 µL of the mixture and 200 µL of the bacterial strain were added to 3 mL of 0.7% agar and poured onto agar plates. The plates were incubated for 8 h at 37 °C. AAS was determined as the rate of phage inactivation K (K = 2.3 × (D/T) × log (P0/Pt), where D is the reciprocal of the serum dilution, T is the phage–serum reaction time (30 min), P0 is the phage titer at the beginning of the phage–serum reaction and Pt is the phage titer after time T of the phage–serum reaction). A rate K of less than 5 was considered to be a low level of phage inactivation, a K of between 5 and 18 as a medium level of phage inactivation and above 18 as a high level of phage inactivation.
A statistical analysis for K rate was performed using the Wilcoxon rank sum test (dependent trials). p < 0.05 was considered significant.
2.4. Categories of the Results of PT
The outcome of PT was assessed according to Międzybrodzki et al. (2012) [].
Categories A–C were recommended as positive responses to PT:
A—pathogen eradication and/or recovery (eradication confirmed by the results of bacterial cultures; recovery refers to wound healing or complete subsidence of the infection symptoms); B—good clinical result (almost complete subsidence of some infection symptoms, together with a significant improvement of the patient’s general condition after completion of PT); C—clinical improvement (discernible reduction in the intensity of some infection symptoms after completion of PT to a degree not observed before PT, when no treatment was used).
Categories D–G were recommended as inadequate responses to PT:
D—questionable clinical improvement (reduction in the intensity of some infection symptoms to a degree that could also be observed before PT); E—transient clinical improvement (reduction in the intensity of some infection symptoms observed only during application of phage preparations and not after termination of PT); F—no response to treatment (lack of reduction in the intensity of some infection symptoms observed before PT); G—clinical deterioration (exacerbation of symptoms of infection at the end of PT).
3. Results
Antiphage activity of sera in 15 patients with chronic urinary or urogenital infections was examined before, during and after PT. The control of AAS was sera from 10 healthy donors showing a low level of AAS against phages: coli 48/D1 (mean K rate = 0.01 ± 0.02), Kl 16/30 (mean K rate = 0.01 ± 0.02), Entc 15/P (mean K rate = 0.07 ± 0.08) and Ps F8/PsF8 (mean K rate = 0.11 ± 0.19).
Fifteen patients using intravaginal and intravesical or intravesical phages received one cycle of 3 days of PT (7 women and 4 men) or the first cycle of 3 days of PT (4 women) (Table 1). Before phage therapy, these patients had low AAS levels (mean K rate = 0.13 ± 0.43). A low level of antibodies in this group of patients was demonstrated on the third day of phage therapy (mean K rate = 0.15 ± 0.51). In this group of patients, the increase in the K rate during PT compared to the K rate before PT was statistically insignificant (Wilcoxon test; p = 0.10). In the period of 14–61 days after the therapy, the level of antibodies was still low (mean K rate = 0.12 ± 0.42). Four women from the first group, who underwent the first cycle of PT, qualified for the second cycle of PT after a 20–61 day break. Four women who had two cycles of PT, before the second cycle of therapy, had low AAS levels (mean K rate = 0.36 ± 0.78). (Table 2). In the second cycle of intravaginal and intravesical or intravesical therapy, the level of AAS on the third day of phage administration was still low (mean K rate = 0.49 ± 0.97). Nevertheless, the increase in the K rate during PT compared to the K rate before PT was statistically significant (Wilcoxon test; p = 0.04).
Analysis of clinical outcome of PT was performed in 11 women with intravaginal and intravesical or intravesical application of phages and in 4 men with intravesical application of phages. Eleven women underwent one cycle of PT or the first cycle of PT intravaginally and intravesically or only intravesically and six of them (54.5%) had A–C results of PT. Four men underwent one cycle of PT intravesically and all men had F results of PT. The results of bacteriological assays prior to, during and after PT are depicted in Table 1 and Table 2. No significant side effects of PT were noted.
4. Discussion
Our recent review presents the current status and perspectives of PT []. In the past decade, interest in PT has rapidly grown and reports on its successful use in treating multidrug-resistant bacterial infections are published almost every month [,,,,,,,,]. Likewise, several reviews on PT are published each year. However, there is a growing gap between the expansion of PT carried out as compassionate use (experimental therapy) and the lack of reliable data derived from clinical trials of PT performed according to the required standards of Evidence-Based Medicine and EMA (FDA). Notably, no double-blind randomized clinical trial has provided conclusive data confirming the real therapeutic value of the therapy. Therefore, there is an urgent need for a well-planned clinical trial that could determine whether indeed PT can offer a reliable weapon against antibiotic-resistant bacterial infections.
The route of phage administration and neutralizing antibody production against phages constitute major factors which may determine the success of such a trial. Our present report extends our earlier data indicating that the production of anti-phage antibodies depends on the route of phage administration []. In fact, intravesical phage administration induced only minimal humoral responses. Those responses increased significantly during repeat phage administration although their level remained low, especially when compared to levels reached in response to topical (e.g., intrawound and intrafistular administration [,]. Furthermore, only weak anti-phage antibody production combined with a lack of significant side effects and good results of the therapy in some patients with UTI appears to be promising.
In conclusion, we report safety and low immunogenicity of intravesicular PT, which suggests that further clinical trials using this approach are warranted.
Author Contributions
S.L. conceived the study, conducted phage therapy and wrote parts of the manuscript; M.Ł.-S. designed and performed the experiments and analyzed data; R.M. analyzed data; M.Ż. designed and performed the experiments and analyzed data; B.W.-D. wrote parts of the manuscript; A.G. conceived the study and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Bioethics Committee at the Wroclaw Medical University (protocol title “Phage inactivation by sera from patients with bacterial infections undergoing phage therapy and healthy subjects”, approval No. KB-414/2014 with further amendments) on 24 June 2014. Phage therapy was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Bioethics Committee at the Wroclaw Medical University (title of the therapeutic experiment project “Experimental phage therapy of antibiotic therapy-resistant infections, including MRSA infections”, approval No. KB-349/2005) on 15 June 2005.
Informed Consent Statement
Informed consent was obtained from all subjects involved in the study.
Data Availability Statement
The data presented in this study are derived from personal patients’ medical records maintained at the Phage Therapy Unit of the Medical Centre as well as Bacteriophage Laboratory of the Institute of Immunology and Experimental Therapy Polish Academy of Sciences in Wrocław, Poland. Those data are not publicly available due to privacy and legal issues (The General Data Protection Regulation (EU) 2016/679 and Act on the rights of the patient and the Patient’s Rights Ombudsman from 6 November 2008).
Acknowledgments
This work was supported by statutory funds of the Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences. We would like to thank Justyna Rękas for her help in preparing a revised version of the manuscript.
Conflicts of Interest
R.M., B.W.-D. and A.G. are co-inventors of patents owned by the Hirszfeld Institute of Immunology and Experimental Therapy and covering phage preparations. S.L., M.Ż. and M.Ł.-S. declare that have no conflict of interest.
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