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Article

Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model

1
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
2
PYCC—Portuguese Yeast Culture Collection, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
3
CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(1), 21; https://doi.org/10.3390/antibiotics10010021
Received: 24 November 2020 / Revised: 12 December 2020 / Accepted: 23 December 2020 / Published: 29 December 2020
(This article belongs to the Special Issue Natural Antimicrobials and Alternatives to Antimicrobials)
The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified. View Full-Text
Keywords: oral microbiota; biofilm; essential oils; antimicrobial activity; toothbrush model oral microbiota; biofilm; essential oils; antimicrobial activity; toothbrush model
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MDPI and ACS Style

Aires, A.; Barreto, A.S.; Semedo-Lemsaddek, T. Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model. Antibiotics 2021, 10, 21. https://doi.org/10.3390/antibiotics10010021

AMA Style

Aires A, Barreto AS, Semedo-Lemsaddek T. Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model. Antibiotics. 2021; 10(1):21. https://doi.org/10.3390/antibiotics10010021

Chicago/Turabian Style

Aires, Andreia, António S. Barreto, and Teresa Semedo-Lemsaddek. 2021. "Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model" Antibiotics 10, no. 1: 21. https://doi.org/10.3390/antibiotics10010021

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