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Open AccessArticle

Energy Transfer between Tm-Doped Upconverting Nanoparticles and a Small Organic Dye with Large Stokes Shift

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Department of Chemistry (Physical Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany
2
Department of Chemistry (Organic Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(1), 9; https://doi.org/10.3390/bios9010009
Received: 29 November 2018 / Revised: 20 December 2018 / Accepted: 28 December 2018 / Published: 8 January 2019
(This article belongs to the Special Issue FRET-Based Biosensors)
Lanthanide-doped upconverting nanoparticles (UCNP) are being extensively studied for bioapplications due to their unique photoluminescence properties and low toxicity. Interest in RET applications involving UCNP is also increasing, but due to factors such as large sizes, ion emission distributions within the particles, and complicated energy transfer processes within the UCNP, there are still many questions to be answered. In this study, four types of core and core-shell NaYF4-based UCNP co-doped with Yb3+ and Tm3+ as sensitizer and activator, respectively, were investigated as donors for the Methyl 5-(8-decanoylbenzo[1,2-d:4,5-d′]bis([1,3]dioxole)-4-yl)-5-oxopentanoate (DBD-6) dye. The possibility of resonance energy transfer (RET) between UCNP and the DBD-6 attached to their surface was demonstrated based on the comparison of luminescence intensities, band ratios, and decay kinetics. The architecture of UCNP influenced both the luminescence properties and the energy transfer to the dye: UCNP with an inert shell were the brightest, but their RET efficiency was the lowest (17%). Nanoparticles with Tm3+ only in the shell have revealed the highest RET efficiencies (up to 51%) despite the compromised luminescence due to surface quenching. View Full-Text
Keywords: resonance energy transfer; DBD dye; core shell UCNP; time-resolved luminescence resonance energy transfer; DBD dye; core shell UCNP; time-resolved luminescence
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MDPI and ACS Style

López de Guereñu, A.; Bastian, P.; Wessig, P.; John, L.; Kumke, M.U. Energy Transfer between Tm-Doped Upconverting Nanoparticles and a Small Organic Dye with Large Stokes Shift. Biosensors 2019, 9, 9.

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