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Biosensors 2019, 9(1), 6; https://doi.org/10.3390/bios9010006

Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor

1
Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
2
Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
*
Authors to whom correspondence should be addressed.
Received: 17 November 2018 / Revised: 12 December 2018 / Accepted: 24 December 2018 / Published: 30 December 2018
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Abstract

A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol–ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis. View Full-Text
Keywords: biofunctionalization; UV light photocatalysis; half antibodies; silicon on insulator; nanophotonic sensor biofunctionalization; UV light photocatalysis; half antibodies; silicon on insulator; nanophotonic sensor
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Sabek, J.; Torrijos-Morán, L.; Griol, A.; Díaz Betancor, Z.; Bañuls Polo, M.-J.; Maquieira, Á.; García-Rupérez, J. Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor. Biosensors 2019, 9, 6.

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