Next Article in Journal
Metal-Organic Frameworks for the Development of Biosensors: A Current Overview
Previous Article in Journal
In Vitro Wounding Models Using the Electric Cell-Substrate Impedance Sensing (ECIS)-Zθ Technology
Article Menu

Export Article

Open AccessArticle
Biosensors 2018, 8(4), 91;

Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection

Istituto per i Processi Chimico Fisici, UOS Bari, Via Orabona 4, 70126 Bari, Italy
Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento, S.P. Lecce-Monteroni, 73100 Lecce, Italy
IZS Puglia e Basilicata, U.O. Putignano. Via Chiancolla 1, C.da. S. Pietro Piturno, 70017 Putignano (BA), Italy
Department of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780 Athens, Greece
Dipartimento di Beni Culturali, Università del Salento, S.P. Lecce-Monteroni, 73100 Lecce, Italy
Dipartimento di Chimica, Università di Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, Italy
Author to whom correspondence should be addressed.
Received: 19 July 2018 / Revised: 13 September 2018 / Accepted: 12 October 2018 / Published: 16 October 2018
(This article belongs to the Special Issue Printed and Flexible Sensors)
Full-Text   |   PDF [4635 KB, uploaded 16 October 2018]   |  


The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed. View Full-Text
Keywords: screen printed electrodes; laser printing; LIFT; glucose; biosensor screen printed electrodes; laser printing; LIFT; glucose; biosensor

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Milano, F.; Giotta, L.; Chirizzi, D.; Papazoglou, S.; Kryou, C.; De Bartolomeo, A.; De Leo, V.; Guascito, M.R.; Zergioti, I. Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection. Biosensors 2018, 8, 91.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top