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Biosensors 2018, 8(2), 27;

Advances in Enzyme-Based Biosensors for Pesticide Detection

National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296 Splaiul Independentei, 060031 Bucharest, Romania
Faculty of Food Engineering, Tourism and Environmental Protection, “Aurel Vlaicu” University of Arad, Elena Dragoi, No. 2, 310330 Arad, Romania
BAE Laboratory, Université de Perpignan via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan, France
International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania
Author to whom correspondence should be addressed.
Received: 28 February 2018 / Revised: 18 March 2018 / Accepted: 20 March 2018 / Published: 22 March 2018
(This article belongs to the Special Issue Electrochemical (Bio)sensors for Environmental and Food Analyses)
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The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more “biologically relevant” than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides. View Full-Text
Keywords: pesticide; enzyme inhibition; biosensor pesticide; enzyme inhibition; biosensor

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Bucur, B.; Munteanu, F.-D.; Marty, J.-L.; Vasilescu, A. Advances in Enzyme-Based Biosensors for Pesticide Detection. Biosensors 2018, 8, 27.

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