High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Laurent A. Francis
Biosensors 2017, 7(1), 12; https://doi.org/10.3390/bios7010012
Received: 13 January 2017
/
Revised: 17 February 2017
/
Accepted: 26 February 2017
/
Published: 1 March 2017
(This article belongs to the Special Issue Systems for the Early Detection of Pathogenic Bacteria)
Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture.
View Full-Text
Keywords:
C-type lectin; agglutination; CRD; bacterial detection; E. coli
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Gasmi, L.; Ferré, J.; Herrero, S. High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae). Biosensors 2017, 7, 12. https://doi.org/10.3390/bios7010012
AMA Style
Gasmi L, Ferré J, Herrero S. High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae). Biosensors. 2017; 7(1):12. https://doi.org/10.3390/bios7010012
Chicago/Turabian StyleGasmi, Leila, Juan Ferré, and Salvador Herrero. 2017. "High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)" Biosensors 7, no. 1: 12. https://doi.org/10.3390/bios7010012
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
