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Open AccessArticle

Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing

1
Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Science, Faculty of Sciences, University of Geneva, 10 route de Suisse, Versoix CH-1290, Switzerland
2
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Biosensors 2015, 5(2), 319-336; https://doi.org/10.3390/bios5020319
Received: 13 April 2015 / Revised: 31 May 2015 / Accepted: 5 June 2015 / Published: 15 June 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in two-dimensional (2D) close-packed arrays. An electric field of 100 V·cm−1, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species (ROS) production and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), and diuron was explored. The results showed significant increase of the cellular ROS when C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and 10−5 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly impacted environment. View Full-Text
Keywords: whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii
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Siebman, C.; Velev, O.D.; Slaveykova, V.I. Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing. Biosensors 2015, 5, 319-336.

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