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Biosensors for Inorganic and Organic Arsenicals
Open AccessArticle

High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide

School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
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Biosensors 2014, 4(4), 513-522; https://doi.org/10.3390/bios4040513
Received: 10 November 2014 / Revised: 18 November 2014 / Accepted: 8 December 2014 / Published: 15 December 2014
Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and the fluorescence emission polarized perpendicular to the polarization of the excitation light was detected. The image contrast with this optical system was improved 110-fold for rhodamine B spotted on the OIM, in comparison with a glass slide using a general fluorescence microscopy optical system. Moreover, a 24-fold improvement of the image contrast was achieved for the detection of Cy3-labeled streptavidin bound to immobilize biotin. View Full-Text
Keywords: fluorescence microscopy; fluorescence enhancement; biochip fluorescence microscopy; fluorescence enhancement; biochip
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Yasuda, M.; Akimoto, T. High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide. Biosensors 2014, 4, 513-522.

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