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Open AccessArticle

Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

1
Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), Branch Bioanalytics and Bioprocesses Potsdam-Golm, Am Mühlenberg 13, 14476 Potsdam, Germany
2
Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
3
Institute for Thin Film and Microsensoric Technology, Kantstr. 55, 14513 Teltow, Germany
4
Biosystems Technology, Institute of Applied Life Sciences, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany
*
Authors to whom correspondence should be addressed.
Biosensors 2014, 4(4), 370-386; https://doi.org/10.3390/bios4040370
Received: 31 July 2014 / Revised: 18 September 2014 / Accepted: 14 October 2014 / Published: 22 October 2014
Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose. View Full-Text
Keywords: multiwalled carbon nanotubes; polyaniline; nanohybrids; cellobiose dehydrogenase multiwalled carbon nanotubes; polyaniline; nanohybrids; cellobiose dehydrogenase
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Tanne, J.; Kracher, D.; Dietzel, B.; Schulz, B.; Ludwig, R.; Lisdat, F.; Scheller, F.W.; Bier, F.F. Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes. Biosensors 2014, 4, 370-386.

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