Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches
Abstract
:1. Introduction
2. Experimental Section
2.1. Reagents and Cells
2.2. Surface Engineering and Lectin Sensor Functionalization
2.3. Electrochemical Impedance Spectroscopy (EIS) Analysis
2.4. Quartz Crystal Microbalance (QCM) Analysis
3. Results and Discussion
3.1. Langmuir Isotherm and Determination
3.2. Lectin Biosensor Construction
3.3. Comparison of Obtained by EIS and QCM
[NB4] (cell/mL) | ∆Rct (Ω/cm2) | [NB4]/∆Rct (cell·cm2/mL·Ω) |
---|---|---|
1 × 103 | 186 ± 72 | 05.81 ± 2.25 |
5 × 103 | 273 ± 83 | 19.33 ± 5.05 |
1 × 105 | 397 ± 55 | 254.81 ± 34.05 |
1 × 106 | 467 ± 54 | 2162.44 ± 270.96 |
[NB4] | Δf (Hz) | [NB4]/Δf (cell/mL·Hz) ×105 |
---|---|---|
1.0 × 105 | 05.93 ± 0.89 | 0.17 ± 0.02 |
2.5 × 106 | 16.39 ± 1.39 | 1.54 ± 0.13 |
5.0 × 106 | 17.83 ± 0.07 | 2.80 ± 0.01 |
1.0 × 107 | 18.05 ± 0.01 | 5.54 ± 0.01 |
1.5 × 107 | 22.50 ± 0.01 | 6.67 ± 0.01 |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Carvalho, F.C.; Martins, D.C.; Santos, A.; Roque-Barreira, M.-C.; Bueno, P.R. Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches. Biosensors 2014, 4, 358-369. https://doi.org/10.3390/bios4040358
Carvalho FC, Martins DC, Santos A, Roque-Barreira M-C, Bueno PR. Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches. Biosensors. 2014; 4(4):358-369. https://doi.org/10.3390/bios4040358
Chicago/Turabian StyleCarvalho, Fernanda C., Denise C. Martins, Adriano Santos, Maria-Cristina Roque-Barreira, and Paulo R. Bueno. 2014. "Evaluating the Equilibrium Association Constant between ArtinM Lectin and Myeloid Leukemia Cells by Impedimetric and Piezoelectric Label Free Approaches" Biosensors 4, no. 4: 358-369. https://doi.org/10.3390/bios4040358