Next Article in Journal / Special Issue
Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
Previous Article in Journal
Comparison of Surface Plasmon Resonance, Resonant Waveguide Grating Biosensing and Enzyme Linked Immunosorbent Assay (ELISA) in the Evaluation of a Dengue Virus Immunoassay
Previous Article in Special Issue
The Effect of 3-Thiopheneacetic Acid in the Polymerization of a Conductive Electrotextile for Use in Biosensor Development
Open AccessArticle

A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform

Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
Biosensors 2013, 3(3), 312-326; https://doi.org/10.3390/bios3030312
Received: 24 July 2013 / Revised: 28 August 2013 / Accepted: 5 September 2013 / Published: 13 September 2013
(This article belongs to the Special Issue Nanomaterials for Biodetection and Drug Delivery)
Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms. View Full-Text
Keywords: surface enhanced Raman scattering; self-referencing; microorganism; nanoparticles surface enhanced Raman scattering; self-referencing; microorganism; nanoparticles
Show Figures

Figure 1

MDPI and ACS Style

Xiao, N.; Wang, C.; Yu, C. A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform. Biosensors 2013, 3, 312-326.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop